ArticlePlant physiology2013
Rapid ammonia gas transport accounts for futile transmembrane cycling under NH3/NH4+ toxicity in plant roots.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it, 130 citations in OpenAlex.
- Plant ammonium sensitivity is associated with external pH adaptation, repertoire of nitrogen transporters, and nitrogen requirement.Journal of experimental botany · 2024Pooled it
- Real-time determination of swine manure liquid compost maturity by oxidation-reduction potential and pH monitoring.Journal of animal science and technology · 2026Article
- Root system architecture modulates nitrogen uptake and use efficiency, influencing grain yield in rice.Scientific reports · 2026Article
- Nitrogen Management in Crop-Soil-Environment Systems: Pathways Toward Sustainable and Climate-Resilient Agriculture.International journal of molecular sciences · 2026Review
- Ammonium assimilation inhibitors weaken the growth of moso bamboo seedlings through metabolic dysregulation.PeerJ · 2026Article
- Overexpression of pxAlaAT3 in Populus × xiaohei alleviates root growth inhibition under ammonium nitrogen stress.Plant cell reports · 2025Article
- Enhancing Crop Nitrogen Efficiency: The Role of Mixed Nitrate and Ammonium Supply in Plant Growth and Development.Biology · 2025Review
- Phenotype Assessment and Putative Mechanisms of Ammonium Toxicity to Plants.International journal of molecular sciences · 2025Review
- Increasing the nitrate-to-ammonium ratio improved plant growth and nitrogen uptake in pineapple seedlings.Frontiers in plant science · 2025Article
- Towards Sustainable Productivity of Greenhouse Vegetable Soils: Limiting Factors and Mitigation Strategies.Plants (Basel, Switzerland) · 2024Review
- Physiological, molecular, and environmental insights into plant nitrogen uptake, and metabolism under abiotic stresses.The plant genome · 2024Review
- Regulating Effect of Exogenous α-Ketoglutarate on Ammonium Assimilation in Poplar.Molecules (Basel, Switzerland) · 2024Article
- Effects of nitrogen application on ammonium assimilation and microenvironment in the rhizosphere of drip-irrigated sunflower under plastic mulch.Frontiers in microbiology · 2024Article
- Plant-microbe interactions in the rhizosphere for smarter and more sustainable crop fertilization: the case of PGPR-based biofertilizers.Frontiers in microbiology · 2024Review
- Plastid-localized amino acid metabolism coordinates rice ammonium tolerance and nitrogen use efficiency.Nature plants · 2023Article
- Effects of Different Nitrogen Forms on Blackberry Fruit Quality.Foods (Basel, Switzerland) · 2023Article
- Review
- Genome-wide identification of resistance genes and transcriptome regulation in yeast to accommodate ammonium toxicity.BMC genomics · 2022Article
- Transporter networks can serve plant cells as nutrient sensors and mimic transceptor-like behavior.iScience · 2022Article
- Nitrogen Source Matters: High NHFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Futile transmembrane NH3/NH4(+) cycling in plant root cells, characterized by extremely rapid fluxes and high efflux to influx ratios, has been successfully linked to NH3/NH4(+) toxicity. Surprisingly, the fundamental question of which species of the conjugate pair (NH3 or NH4(+)) participates in such fluxes is unresolved. Using flux analyses with the short-lived radioisotope (13)N and electrophysiological, respiratory, and histochemical measurements, we show that futile cycling in roots of barley (Hordeum vulgare) seedlings is predominately of the gaseous NH3 species, rather than the NH4(+) ion. Influx of (13)NH3/(13)NH4(+), which exceeded 200 µmol g(-1) h(-1), was not commensurate with membrane depolarization or increases in root respiration, suggesting electroneutral NH3 transport. Influx followed Michaelis-Menten kinetics for NH3 (but not NH4(+)), as a function of external concentration (Km = 152 µm, Vmax = 205 µmol g(-1) h(-1)). Efflux of (13)NH3/(13)NH4(+) responded with a nearly identical Km. Pharmacological characterization of influx and efflux suggests mediation by aquaporins. Our study fundamentally revises the futile-cycling model by demonstrating that NH3 is the major permeating species across both plasmalemma and tonoplast of root cells under toxicity conditions.
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