ArticleAnalytical chemistry2013
Mass spectrometry imaging for dissecting steroid intracrinology within target tissues.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 124 citations in OpenAlex.
- Visualization of relative 11β-hydroxylase (CYP11B1) activity in human cortisol-producing adrenocortical adenoma (CPA) using mass spectrometry imaging-based enzyme histochemistry.Analytical and bioanalytical chemistry · 2026Article
- Chemical Derivatization Strategies for Expanding Small Biomolecule Analysis by MALDI-MS.Metabolites · 2026Review
- Platinum thin film-based matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry imaging for improved ion yields of adrenocortical steroids.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Article
- Spatial lipidomic combining DESI imaging and LESA mass spectrometry to evaluate metabolic alterations in obese zebrafish.Journal of lipid research · 2026Article
- Localisation of corticosteroids in male mouse kidney by mass spectrometry imaging.Journal of molecular endocrinology · 2026Article
- Advanced multi-modal mass spectrometry imaging reveals functional differences of placental villous compartments at microscale resolution.Nature communications · 2025Article
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Lipopolysaccharide differentially alters systemic and brain glucocorticoid levels in neonatal and adult mice.Journal of neuroendocrinology · 2025Article
- Tissue derivatization for visualizing lactate and pyruvate in mouse testis tissues using matrix-assisted laser desorption/ionization-mass spectrometry imaging.Analytical and bioanalytical chemistry · 2024Article
- Spatial neurolipidomics-MALDI mass spectrometry imaging of lipids in brain pathologies.Journal of mass spectrometry : JMS · 2024Review
- Mass Spectrometry Imaging of Cholesterol and Oxysterols.Advances in experimental medicine and biology · 2024Article
- Revealing Structure and Localization of Steroid Regioisomers through Predictive Fragmentation Patterns in Mass Spectrometry Imaging.Analytical chemistry · 2023Article
- High-resolution ion mobility spectrometry-mass spectrometry for isomeric separation of prostanoids after Girard's reagent T derivatization.Rapid communications in mass spectrometry : RCM · 2023Article
- Molecular Networking and On-Tissue Chemical Derivatization for Enhanced Identification and Visualization of Steroid Glycosides by MALDI Mass Spectrometry Imaging.Analytical chemistry · 2022Article
- Spatial localization of β-unsaturated aldehyde markers in murine diabetic kidney tissue by mass spectrometry imaging.Analytical and bioanalytical chemistry · 2022Article
- On-tissue chemical derivatization in mass spectrometry imaging.Mass spectrometry reviews · 2022Review
- Rapidity and Precision of Steroid Hormone Measurement.Journal of clinical medicine · 2022Review
- Applications of spatially resolved omics in the field of endocrine tumors.Frontiers in endocrinology · 2022Review
- Glucocorticoid production in the thymus and brain: Immunosteroids and neurosteroids.Brain, behavior, & immunity - health · 2021Article
- Spatial distribution of isobaric androgens in target tissues using chemical derivatization and MALDI-2 on a trapped ion mobility quadrupole time-of-flight instrument.RSC advances · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Steroid concentrations within tissues are modulated by intracellular enzymes. Such "steroid intracrinology" influences hormone-dependent cancers and obesity and provides targets for pharmacological inhibition. However, no high resolution methods exist to quantify steroids within target tissues. We developed mass spectrometry imaging (MSI), combining matrix assisted laser desorption ionization with on-tissue derivatization with Girard T and Fourier transform ion cyclotron resonance mass spectrometry, to quantify substrate and product (11-dehydrocorticosterone and corticosterone) of the glucocorticoid-amplifying enzyme 11β-HSD1. Regional steroid distribution was imaged at 150-200 μm resolution in rat adrenal gland and mouse brain sections and confirmed with collision induced dissociation/liquid extraction surface analysis. In brains of mice with 11β-HSD1 deficiency or inhibition, MSI quantified changes in subregional corticosterone/11-dehydrocorticosterone ratio, distribution of inhibitor, and accumulation of the alternative 11β-HSD1 substrate, 7-ketocholesterol. MSI data correlated well with LC-MS/MS in whole brain homogenates. MSI with derivatization is a powerful new tool to investigate steroid biology within tissues.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.