ArticleInternational journal of clinical and experimental pathology2013
Assessment of different decalcifying protocols on Osteopontin and Osteocalcin immunostaining in whole bone specimens of arthritis rat model by confocal immunofluorescence.
Article in International journal of clinical and experimental pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Rapamycin reveals neuropeptide Y as a regulator of senescence and inflammatory pathways in arthritis.Neuropeptides · 2025Article
- Qualitative comparison of decalcifiers for mouse bone cryosections for subsequent biophotonic analysis.Scientific reports · 2025Article
- Effect of vitamin D deficiency on postorthodontic relapse: An animal study.Clinical and experimental dental research · 2023Article
- Whole-mouse clearing and imaging at the cellular level with vDISCO.Nature protocols · 2023Review
- Article
- Article
- Scalable tissue labeling and clearing of intact human organs.Nature protocols · 2022Review
- Effects of Chronic Oral Probiotic Treatment in Paclitaxel-Induced Neuropathic Pain.Biomedicines · 2021Article
- Positive transcriptional response on inflammation and joint remodelling influenced by physical exercise in proteoglycan-induced arthritis: An animal study.Bone & joint research · 2020Article
- Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society.Frontiers in endocrinology · 2020Review
- Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma.Biochemistry research international · 2020Article
- Article
- Anatomy and function of the vertebral column lymphatic network in mice.Nature communications · 2019Article
- Tissue Morphology and Antigenicity in Mouse and Rat Tibia: Comparing 12 Different Decalcification Conditions.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone.Journal of visualized experiments : JoVE · 2019Article
- Rat Model of Adhesive Capsulitis of the Shoulder.Journal of visualized experiments : JoVE · 2018Article
- Pathologic conditions of hard tissue: role of osteoclasts in osteolytic lesion.Histochemistry and cell biology · 2018Review
- Comparison of Different Decalcification Methods Using Rat Mandibles as a Model.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
- Evaluation of Decalcification Techniques for Rat Femurs Using HE and Immunohistochemical Staining.BioMed research international · 2017Article
- Ellagic acid alleviates adjuvant induced arthritis by modulation of pro- and anti-inflammatory cytokines.Central-European journal of immunology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Confocal immunofluorescence is a valuable technique for the detection of relevant molecules in the pathogenesis of arthritis in rat models; however, it requires efficient processing of tissues including bone decalcification. The decalcification process must ensure the complete removal of calcium and also a proper preservation of cellular structures and, specially, the antigenicity of the tissue to allow the immunodetection of the molecules of interest. In the present study, we evaluated the effect of four different decalcifying solutions: the Morse´s solution, 10% EDTA (pH 7.4), 7% HCl/2% EDTA and 5% Nitric acid, as well as four different treatments of the tissues (including microwave irradiation) in the processes of decalcification for large pieces of adult rat bones (hind paw, fore paw, knee and column). We assessed the time of decalcification, the easiness of slicing, the morphological preservation and finally, the antigenicity of two different bone proteins (Osteopontin (OPN) and Osteocalcin (OC)) measured by its immunofluorescence intensity under controlled confocal microscopy conditions. Our results showed that the specimen size and the presence of skin are critical factors for the rate of decalcification, and no significant benefit was found if microwave irradiation is applied to the tissue. The comprehensive statistical analysis showed that the optimal solution for the detection of OPN and OC by confocal immunofluorescence is the 5% Nitric Acid, and followed by 10% EDTA (pH 7.4), Ana Morse solution and 7% HCl/2% EDTA.
Indexed as
Identifiers
24133575PMC3796219W67567543What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.