ArticleRadiology and oncology2013
The potential value of the neutral comet assay and γH2AX foci assay in assessing the radiosensitivity of carbon beam in human tumor cell lines.
Article in Radiology and oncology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 28 citations in OpenAlex.
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- Combined pretreatment withTrials · 2018Article
- Comparing Photon and Charged Particle Therapy Using DNA Damage Biomarkers.International journal of particle therapy · 2018Review
- pATM and γH2AX are effective radiation biomarkers in assessing the radiosensitivity ofCancer cell international · 2017Article
- Influence of selected anti-cancer drugs on the induction of DNA double-strand breaks and changes in gene expression in human hepatoma HepG2 cells.Environmental science and pollution research international · 2016Article
- Multiscale approach predictions for biological outcomes in ion-beam cancer therapy.Scientific reports · 2016Article
- Article
- Expression of the γ-phosphorylated histone H2AX in gastric carcinoma and gastric precancerous lesions.Oncology letters · 2015Article
- Proteomic analysis of effects by x-rays and heavy ion in HeLa cells.Radiology and oncology · 2014Article
- Induction of H2AX phosphorylation in tumor cells by gossypol acetic acid is mediated by phosphatidylinositol 3-kinase (PI3K) family.Cancer cell international · 2014Article
- Radio-Protective Effect of Lanzhou Lily Polysaccharide on the Spleen in Mice.Dose-response : a publication of International Hormesis SocietyArticle
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8 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCarbon ions ((12)C(6+)) are high linear energy transfer (LET) radiation characterized by higher relative biological effectiveness than low LET radiation. The assessment of tumour radiosensitivity would be particularly useful in optimizing the radiation dose during radiotherapy. The aim of the current study was to evaluate the potential value of the neutral comet assay and γH2AX foci assay in assessing (12)C(6+) radiosensitivity of tumour cells. MATERIALS AND
methodsThe doses of (12)C(6+) and X-rays used in the present study were 2 and 4 Gy. The survival fraction, DNA double-strand breaks (DSB) and repair kinetics of DSB were assayed with clonogenic survival, neutral comet assay and γH2AX foci assay in human cervical carcinoma HeLa cells, hepatoma HepG2 cells, and mucoepidermoid carcinoma MEC-1 cells at the time points of 0.5, 4, 16 and 24 h after (12)C(6+) and X-rays irradiation.
resultsThe survival fraction for 12C6+ irradiation was much more inhibited than for X-rays (p < 0.05) in all three tumour cell lines tested. Substantial amounts of residual damage, assessed by the neutral comet assay, were present after irradiation (p < 0.05). The highest residual damage was observed at 0.5 or 4 h, both for (12)C(6+) and X-ray irradiation. However, the residual damage in HeLa and MEC-1 cells was higher for (12)C(6+) than X-rays (p < 0.05). The strongest induction of γH2AX foci was observed after 30 min, for all three tumour cell lines (p < 0.01). The franction of γH2AX foci persisted for at least 24 h after (12)C6+ irradiation; in HeLa cells and MEC-1 was higher than after X-ray irradiation (p < 0.05). The correlation coefficients between the clonogenic survival, neutral comet assay and γH2AX foci assay were not statistically significant, except for some tumour cells at individual irradiation doses and types.
conclusionsOur study demonstrated that the neutral comet assay and γ-H2AX foci assay could be used to assess the radiosensitivity of (12)C(6+) in human tumour cells.
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