ArticleThe Journal of biological chemistry2013
Distinct roles of the phosphatidate phosphatases lipin 1 and 2 during adipogenesis and lipid droplet biogenesis in 3T3-L1 cells.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- snRNA-seq of adipose tissues reveals the potential cellular and molecular mechanisms of cold and disease resistance in Mongolian cattle.BMC genomics · 2024Article
- Liposome-Mediated Anti-Viral Drug Delivery Across Blood-Brain Barrier: Can Lipid Droplet Target Be Game Changers?Cellular and molecular neurobiology · 2023Review
- Monoacylglycerol O-acyltransferase 1 lowers adipocyte differentiation capacity in vitro but does not affect adiposity in mice.Obesity (Silver Spring, Md.) · 2022Article
- Lipid Droplets, Phospholipase ABiomedicines · 2021Review
- Article
- Regulation of Signaling and Metabolism by Lipin-mediated Phosphatidic Acid Phosphohydrolase Activity.Biomolecules · 2020Review
- Purified Gymnemic Acids fromNutrients · 2020Article
- Antiretroviral Therapy-Associated Metabolic Complications: Review of the Recent Studies.HIV/AIDS (Auckland, N.Z.) · 2020Review
- miRNA-218 Targets Lipin-1 and Glucose Transporter Type 4 Genes in 3T3-L1 Cells Treated With Lopinavir/Ritonavir.Frontiers in pharmacology · 2019Article
- New Method for Quantitation of Lipid Droplet Volume From Light Microscopic Images With an Application to Determination of PAT Protein Density on the Droplet Surface.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2018Article
- Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury.Scientific reports · 2018Article
- Synergistic Effect of Bupleuri Radix and Scutellariae Radix on Adipogenesis and AMP-Activated Protein Kinase: A Network Pharmacological Approach.Evidence-based complementary and alternative medicine : eCAM · 2018Article
- Normal human adipose tissue functions and differentiation in patients with biallelicJournal of lipid research · 2017Article
- Lipin proteins and glycerolipid metabolism: Roles at the ER membrane and beyond.Biochimica et biophysica acta. Biomembranes · 2017Review
- Tips on the analysis of phosphatidic acid by the fluorometric coupled enzyme assay.Analytical biochemistry · 2017Article
- Redundant roles of the phosphatidate phosphatase family in triacylglycerol synthesis in human adipocytes.Diabetologia · 2016Article
- In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Lipin-1 regulates cancer cell phenotype and is a potential target to potentiate rapamycin treatment.Oncotarget · 2015Article
- Phospholipase D and phosphatidic acid in plant defence response: from protein-protein and lipid-protein interactions to hormone signalling.Journal of experimental botany · 2015Review
- Lipin 2 binds phosphatidic acid by the electrostatic hydrogen bond switch mechanism independent of phosphorylation.The Journal of biological chemistry · 2014Article
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
Lipins are evolutionarily conserved Mg(2+)-dependent phosphatidate phosphatase (PAP) enzymes with essential roles in lipid biosynthesis. Mammals express three paralogues: lipins 1, 2, and 3. Loss of lipin 1 in mice inhibits adipogenesis at an early stage of differentiation and results in a lipodystrophic phenotype. The role of lipins at later stages of adipogenesis, when cells initiate the formation of lipid droplets, is less well characterized. We found that depletion of lipin 1, after the initiation of differentiation in 3T3-L1 cells but before the loading of lipid droplets with triacylglycerol, results in a reciprocal increase of lipin 2, but not lipin 3. We generated 3T3-L1 cells where total lipin protein and PAP activity levels are down-regulated by the combined depletion of lipins 1 and 2 at day 4 of differentiation. These cells still accumulated triacylglycerol but displayed a striking fragmentation of lipid droplets without significantly affecting their total volume per cell. This was due to the lack of the PAP activity of lipin 1 in adipocytes after day 4 of differentiation, whereas depletion of lipin 2 led to an increase of lipid droplet volume per cell. We propose that in addition to their roles during early adipogenesis, lipins also have a role in lipid droplet biogenesis.
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