Evidence map›Paper›PMID 24132917›Full record

ArticleClinical cancer research : an official journal of the American Association for Cancer Research2014

αB-crystallin: a novel regulator of breast cancer metastasis to the brain.

Dmitry Malin, Elena Strekalova, Vladimir Petrovic, Allison M Deal, Abraham Al Ahmad, Barbara Adamo, C Ryan Miller, Andrey Ugolkov, Chad Livasy, Karen Fritchie and 8 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical cancer research : an official journal of the American Association for Cancer Research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed, 1 pooled it
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed, 1 synthesis or guideline pooled it, 100 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. Critical functions of extracellular matrix in brain metastasis seeding.Cellular and molecular life sciences : CMLS · 2023
    Review
  8. The origin of brain malignancies at the blood-brain barrier.Cellular and molecular life sciences : CMLS · 2023
    Review
  9. Review
  10. Article
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  12. Article
  13. Article
  14. Article
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  20. Advancing human induced pluripotent stem cell-derived blood-brain barrier models for studying immune cell interactions.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 7 institutions in 2 countries.

Dmitry MalinAuthors' Affiliations: Department of Medicine, University of Wisconsin Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health; Department of Chemical and Biological Engineering, University of Wisconsin, Madison, Wisconsin; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Breast Cancer Unit, Medical Oncology Department, Vall d'Hebron Institute of Oncology, Barcelona, Spain; Center for Developmental Therapeutics, Northwestern University, Evanston, Illinois; Department of Pathology and Lab Medicine, Carolinas Medical Center, Charlotte, North Carolina; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota; and Department of Medicine and Department of Pathology, Duke University Medical Center, Durham, North Carolina.
Elena Strekalova
Vladimir Petrovic
Allison M Deal
Abraham Al Ahmad
Barbara Adamo
C Ryan Miller
Andrey Ugolkov
Chad Livasy
Karen Fritchie
Erika Hamilton
Kimberly Blackwell
Joseph Geradts
Matt Ewend
Lisa Carey
Eric V Shusta
Carey K Anders
Vincent L Cryns
Northwestern University · USUniversity of Wisconsin–Madison · USUniversity of North Carolina at Chapel Hill · USCarolinas Medical Center · USVall d'Hebron Hospital Universitari · ESVall d'Hebron Institute of OncologyMayo Clinic · US

Funding

Virology Research Program (Program 4)P30CA016086 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Deborah F. Tate · 1985 to 2026
$201.5M
UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI AMY TRENTHAM-DIETZ · 1985 to 2026
$142.6M
UNC Oncology K12K12CA120780 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI WILLIAM Y. KIM · 2007 to 2026
$9.6M
Tumor Targeted Nanobins for the Treatment of Metastatic Breast and Ovarian CancerU01CA151461 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI O'HALLORAN, THOMAS V · 2010 to 2014
$2.2M
PARP Inhibition to Treat Triple-Negative Breast Cancer Brain MetastasesK23CA157728 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ANDERS, CAREY KERNODLE · 2011 to 2015
$867k
NCI NIH HHS K12 CA120780NCI NIH HHS K23 CA157728NCI NIH HHS P30 CA014520NCI NIH HHS P30 CA016086NCI NIH HHS U01 CA151461
6 · The paper itself

Abstract

purposeBasal-like breast tumors are typically (ER/PR/HER2) triple-negative and are associated with a high incidence of brain metastases and poor clinical outcomes. The molecular chaperone αB-crystallin is predominantly expressed in triple-negative breast cancer (TNBC) and contributes to an aggressive tumor phenotype in preclinical models. We investigated the potential role of αB-crystallin in brain metastasis in TNBCs. EXPERIMENTAL

designαB-crystallin expression in primary breast carcinomas and brain metastases was analyzed by immunohistochemistry among patients with breast cancer with brain metastases. αB-crystallin was overexpressed or silenced in two different TNBC cell lines. The effects on cell adhesion to human brain microvascular endothelial cells (HBMEC) or extracellular matrix proteins, transendothelial migration, and transmigration across a HBMEC/astrocyte coculture blood-brain barrier (BBB) model were examined. In addition, the effects of overexpressing or silencing αB-crystallin on brain metastasis in vivo were investigated using orthotopic TNBC models.

resultsIn a cohort of women with breast cancer brain metastasis, αB-crystallin expression in primary breast carcinomas was associated with poor overall survival and poor survival after brain metastasis, even among patients with TNBC. Stable overexpression of αB-crystallin in TNBC cells enhanced adhesion to HBMECs, transendothelial migration, and BBB transmigration in vitro, whereas silencing αB-crystallin inhibited these events. αB-crystallin promoted adhesion of TNBC cells to HBMECs, at least in part, through an α3β1 integrin-dependent mechanism. αB-crystallin overexpression promoted brain metastasis, whereas silencing αB-crystallin inhibited brain metastasis in orthotopic TNBC models.

conclusionαB-crystallin is a novel regulator of brain metastasis in TNBC and represents a potential biomarker and drug target for this aggressive disease.

Indexed as

alpha-Crystallin B ChainAnimalsBlood-Brain BarrierBrain NeoplasmsCell AdhesionCell Line, TumorEndothelium, VascularFemaleGene ExpressionGene Knockdown TechniquesHumansKaplan-Meier EstimateMice, Inbred NODMice, KnockoutMice, SCIDNeoplasm Transplantationalpha-Crystallin B Chain

Identifiers

PMID24132917
PMCPMC3973485
OpenAlexW2154598403

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.