ArticleClinical and vaccine immunology : CVI2013
Evaluation of a commercial enzyme-linked immunosorbent assay for the diagnosis of bovine tuberculosis from milk samples from dairy cows.
Article in Clinical and vaccine immunology : CVI, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A recombinant BCG vaccine induces specific immunity against respiratory syncytial virus andEmerging microbes & infections · 2026Article
- Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area.Frontiers in veterinary science · 2025Article
- B Cell and Antibody Responses in Bovine Tuberculosis.Antibodies (Basel, Switzerland) · 2024Review
- Effect of a recent intradermal test on the specificity of P22 ELISA for the diagnosis of caprine tuberculosis.Frontiers in veterinary science · 2024Article
- Diagnostic accuracy of the Enferplex Bovine TB antibody test using individual milk samples from cattle.PloS one · 2024Article
- Effect of the topical administration of corticosteroids and tuberculin pre-sensitisation on the diagnosis of tuberculosis in goats.BMC veterinary research · 2022Article
- Effect of a recent parenteral dexamethasone and ketoprofen administration on the immunological diagnosis of tuberculosis in goats.Frontiers in veterinary science · 2022Article
- Serological detection of Mycobacterium Tuberculosis complex infection in multiple hosts by One Universal ELISA.PloS one · 2021Article
- Evaluation of P22 ELISA for the Detection ofFrontiers in veterinary science · 2021Article
- Application of Volatilome Analysis to the Diagnosis of Mycobacteria Infection in Livestock.Frontiers in veterinary science · 2021Review
- Comparing recombinant MPB70/SahH and native 20-kDa protein for detecting bovine tuberculosis using ELISA.The Journal of veterinary medical science · 2020Article
- The possible zoonotic diseases transferring from pig to human in Vietnam.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Bayesian estimation of ELISA and gamma interferon test accuracy for the detection of bovine tuberculosis in caudal fold test-negative dairy cattle in Kuwait.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018Article
- Investigation of the cause of geographic disparities in IDEXX ELISA sensitivity in serum samples from Mycobacterium bovis-infected cattle.Scientific reports · 2016Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Milk samples from dairy cows provide a ready source of material for measuring antibody responses to Mycobacterium bovis antigens. In this study, we evaluated the IDEXX enzyme-linked immunosorbent assay (ELISA) for the measurement of antibody responses to M. bovis antigens MPB70 and MPB83 in milk samples from New Zealand cattle. Test sensitivities for individual milk and serum samples were assessed in samples collected from 44 M. bovis-infected cows, and test specificities were assessed in milk samples collected from 356 cows from tuberculosis (TB)-free herds. Milk vat samples were collected from 505 herds from regions with relatively high or low prevalences of infection. The ELISA had a sensitivity of 50% and a specificity of 97.5% for milk samples, and the test sensitivities for milk and serum samples were the same. Dilution of the positive test milk samples in milk from noninfected cows at 1/10, 1/20, and 1/50 dilutions reduced the proportions of positive responses to 13/21, 9/21, and 4/21, respectively. Small differences were observed in the ELISA responses of milk samples from individual TB-free cows collected at different times during lactation. No significant differences were detected in the ELISA responses of milk vat samples collected from infected and noninfected herds. This study shows that milk samples can be substituted for serum samples for screening individual cows for M. bovis infection, and pooling of milk samples from 10 to 20 animals can result in a reduction in the sensitivity by approximately 50%. However, screening of milk vat samples is unlikely to be useful in countries with low prevalences of M. bovis in cattle and large herd sizes.
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