ArticleInternational journal of molecular sciences2013
Absolute quantification of selected proteins in the human osteoarthritic secretome.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Extracellular matrix composition of connective tissues: a systematic review and meta-analysis.Scientific reports · 2019Pooled it
- Current cutting-edge omics techniques on musculoskeletal tissues and diseases.Bone research · 2025Review
- Computational Translation of Mouse Models of Osteoarthritis Predicts Human Disease.bioRxiv : the preprint server for biology · 2025Article
- Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome After Microfracture or Osteotomy.The American journal of sports medicine · 2021Article
- Impaired chondrocyte U3 snoRNA expression in osteoarthritis impacts the chondrocyte protein translation apparatus.Scientific reports · 2020Article
- The antiviral protein viperin regulates chondrogenic differentiation via CXCL10 protein secretion.The Journal of biological chemistry · 2019Article
- Donor age affects proteome composition of tenocyte-derived engineered tendon.BMC biotechnology · 2018Article
- Systems approaches in osteoarthritis: Identifying routes to novel diagnostic and therapeutic strategies.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017Article
- Comparison between chaotropic and detergent-based sample preparation workflow in tendon for mass spectrometry analysis.Proteomics · 2017Article
- Autologous chondrocyte implantation-derived synovial fluids display distinct responder and non-responder proteomic profiles.Arthritis research & therapy · 2017Article
- Accurate quantification of modified cyclic peptides without the need for authentic standards.Tetrahedron · 2016Article
- A proteomic analysis of chondrogenic, osteogenic and tenogenic constructs from ageing mesenchymal stem cells.Stem cell research & therapy · 2016Article
- Article
- Anatomical heterogeneity of tendon: Fascicular and interfascicular tendon compartments have distinct proteomic composition.Scientific reports · 2016Article
- Characterization of neopeptides in equine articular cartilage degradation.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2016Article
- Aging-related inflammation in osteoarthritis.Osteoarthritis and cartilage · 2015Review
- Human genome-wide expression analysis reorients the study of inflammatory mediators and biomechanics in osteoarthritis.Osteoarthritis and cartilage · 2015Review
- Comprehensive protein profiling of synovial fluid in osteoarthritis following protein equalization.Osteoarthritis and cartilage · 2015Article
- Subchondral bone proteomics in osteoarthritis: Current status and perspectives.Journal of orthopaedic translation · 2015Review
- Transcriptome analysis of ageing in uninjured human Achilles tendon.Arthritis research & therapy · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Osteoarthritis (OA) is characterized by a loss of extracellular matrix which is driven by catabolic cytokines. Proteomic analysis of the OA cartilage secretome enables the global study of secreted proteins. These are an important class of molecules with roles in numerous pathological mechanisms. Although cartilage studies have identified profiles of secreted proteins, quantitative proteomics techniques have been implemented that would enable further biological questions to be addressed. To overcome this limitation, we used the secretome from human OA cartilage explants stimulated with IL-1β and compared proteins released into the media using a label-free LC-MS/MS-based strategy. We employed QconCAT technology to quantify specific proteins using selected reaction monitoring. A total of 252 proteins were identified, nine were differentially expressed by IL-1 β stimulation. Selected protein candidates were quantified in absolute amounts using QconCAT. These findings confirmed a significant reduction in TIMP-1 in the secretome following IL-1β stimulation. Label-free and QconCAT analysis produced equivocal results indicating no effect of cytokine stimulation on aggrecan, cartilage oligomeric matrix protein, fibromodulin, matrix metalloproteinases 1 and 3 or plasminogen release. This study enabled comparative protein profiling and absolute quantification of proteins involved in molecular pathways pertinent to understanding the pathogenesis of OA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.