ArticleBMC complementary and alternative medicine2013
In vitro anti-Herpes simplex virus activity of crude extract of the roots of Nauclea latifolia Smith (Rubiaceae).
Article in BMC complementary and alternative medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed.
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- Phytochemical profiling, antiviral activities, molecular docking, and dynamic simulations of selected Ruellia species extracts.Scientific reports · 2024Article
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- Phytochemical analysis, antioxidant capacities, and in vitro biological activities of the extract of seed coat as by-products of pea.BMC chemistry · 2023Article
- Synthesis of bee venom loaded chitosan nanoparticles for anti-MERS-COV and multi-drug resistance bacteria.International journal of biological macromolecules · 2023Article
- 1,2,3-Triazole-Benzofused Molecular Conjugates as Potential Antiviral Agents against SARS-CoV-2 Virus Variants.Life (Basel, Switzerland) · 2022Article
- The Inhibition of DNA Viruses by the Amphibian Antimicrobial Peptide Temporin G: A Virological Study Addressing HSV-1 and JPCyV.International journal of molecular sciences · 2022Article
- Thymus capitatus flavonoids inhibit infection of Kaposi's sarcoma-associated herpesvirus.FEBS open bio · 2022Article
- Medicinal plants and natural compounds against acyclovir-resistant HSV infections.Frontiers in microbiology · 2022Review
- Anti-Herpes Simplex Virus Efficacy of Silk Cocoon, Silkworm Pupa and Non-Sericin Extracts.Antibiotics (Basel, Switzerland) · 2021Article
- Antiviral, Cytotoxic, and Antioxidant Activities of Three Edible Agaricomycetes Mushrooms:Journal of fungi (Basel, Switzerland) · 2021Article
- Proteome Analysis and In Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts ofMolecules (Basel, Switzerland) · 2021Article
- Progress of Plant Medicine Derived Extracts and Alkaloids on Modulating Viral Infections and Inflammation.Drug design, development and therapy · 2021Review
- Modified cyclodextrins as broad-spectrum antivirals.Science advances · 2020Article
- Model Optimization and In Silico Analysis of Potential Dipeptidyl Peptidase IV Antagonists from GC-MS Identified Compounds inInternational journal of molecular sciences · 2019Article
- The Amphibian Antimicrobial Peptide Temporin B InhibitsAntimicrobial agents and chemotherapy · 2018Article
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12 authors.
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Abstract
backgroundNauclea latifolia Smith, a shrub belonging to the family Rubiaceae is a very popular medicinal plant in Cameroon and neighboring countries where it is used to treat jaundice, yellow fever, rheumatism, abdominal pains, hepatitis, diarrhea, dysentery, hypertension, as well as diabetes. The ethno-medicinal use against yellow fever, jaundice and diarrhea prompted us to investigate on the antiviral activity of the root bark of N. latifolia. In this study, HSV-2 was chosen as a viral model because of its strong impact on HIV transmission and acquisition.
methodsThe crude extract under study was prepared by maceration of air-dried and powdered roots barks of N. latifolia in CH2Cl2/MeOH (50:50) mixture for 48 hours, then it was subjected to filtration and evaporation under vacuum. A phytochemical analysis of the crude extract was performed by High Performance Liquid Chromatography coupled with a photodiode array and mass spectrometry (HPLC-PDA-ESI-qMS). The anti-HSV-2 activity was assayed in vitro by plaque reduction and virus yield assays and the major mechanism of action was investigated by virucidal and time of addition assays. Data values were compared using the Extra sum of squares F test of program GraphPad PRISM 4.
resultsThe main components detected in the extract belong to the class of indole alkaloids characteristic of Nauclea genus. Strictosamide, vincosamide and pumiloside were tentatively identified together with quinovic acid glycoside. N. latifolia crude extract inhibited both acyclovir sensitive and acyclovir resistant HSV-2 strains, with IC50 values of 5.38 μg/ml for the former and 7.17 μg/ml for the latter. The extract was found to be most active when added post-infection, with IC50 of 3.63 μg/ml.
conclusionThe results of this work partly justify the empirical use of N. latifolia in traditional medicine for the treatment of viral diseases. This extract could be a promising rough material for the development of a new and more effective modern anti-HSV-2 medication also active against acyclovir-resistant HSV-2 strains.
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