ArticleBMC biology2013
Requirement for integrin-linked kinase in neural crest migration and differentiation and outflow tract morphogenesis.
Article in BMC biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
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- Whole exome sequencing reveals genetic landscape associated with left ventricular outflow tract obstruction in Chinese Han population.Frontiers in genetics · 2023Article
- Requirement for PINCH in skeletal myoblast differentiation.Cell and tissue research · 2023Article
- Crk and Crkl have shared functions in neural crest cells for cardiac outflow tract septation and vascular smooth muscle differentiation.Human molecular genetics · 2022Article
- Understanding the molecular basis of cardiomyopathy.American journal of physiology. Heart and circulatory physiology · 2022Review
- Cardiac Neural Crest Cells: Their Rhombomeric Specification, Migration, and Association with Heart and Great Vessel Anomalies.Cellular and molecular neurobiology · 2021Review
- Cardiac Neural Crest.Cold Spring Harbor perspectives in biology · 2021Review
- Extracellular Matrix Disparities in anFrontiers in cardiovascular medicine · 2020Article
- Cortical interlaminar astrocytes across the therian mammal radiation.The Journal of comparative neurology · 2019Article
- Beyond cancer genes: colorectal cancer as robust intrinsic states formed by molecular interactions.Open biology · 2017Article
- One crisis, diverse impacts-Tissue-specificity of folate deficiency-induced circulation defects in zebrafish larvae.PloS one · 2017Article
- DNA methylation changes in the placenta are associated with fetal manganese exposure.Reproductive toxicology (Elmsford, N.Y.) · 2015Article
- Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation.The Journal of clinical investigation · 2015Article
- Protein expression profiles characterize distinct features of mouse cerebral cortices at different developmental stages.PloS one · 2015Article
- Mesodermal expression of integrin α5β1 regulates neural crest development and cardiovascular morphogenesis.Developmental biology · 2014Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundNeural crest defects lead to congenital heart disease involving outflow tract malformation. Integrin-linked-kinase (ILK) plays important roles in multiple cellular processes and embryogenesis. ILK is expressed in the neural crest, but its role in neural crest and outflow tract morphogenesis remains unknown.
resultsWe ablated ILK specifically in the neural crest using the Wnt1-Cre transgene. ILK ablation resulted in abnormal migration and overpopulation of neural crest cells in the pharyngeal arches and outflow tract and a significant reduction in the expression of neural cell adhesion molecule (NCAM) and extracellular matrix components. ILK mutant embryos exhibited an enlarged common arterial trunk and ventricular septal defect. Reduced smooth muscle differentiation, but increased ossification and neurogenesis/innervation were observed in ILK mutant outflow tract that may partly be due to reduced transforming growth factor β2 (TGFβ2) but increased bone morphogenetic protein (BMP) signaling. Consistent with these observations, microarray analysis of fluorescence-activated cell sorting (FACS)-sorted neural crest cells revealed reduced expression of genes associated with muscle differentiation, but increased expression of genes of neurogenesis and osteogenesis.
conclusionsOur results demonstrate that ILK plays essential roles in neural crest and outflow tract development by mediating complex crosstalk between cell matrix and multiple signaling pathways. Changes in these pathways may collectively result in the unique neural crest and outflow tract phenotypes observed in ILK mutants.
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