ArticleJournal of virology2014
Functional characterization of bovine viral diarrhea virus nonstructural protein 5A by reverse genetic analysis and live cell imaging.
Article in Journal of virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- GFP-Tagged EViruses · 2026Article
- Pestivirus bovine viral diarrhea virus infection triggers lipophagy through the AMPK-PNPLA2/ATGL signaling pathway to promote viral replication.Autophagy · 2025Article
- Deciphering the membrane topology of the pestiviral non-structural protein 4B (NS4B).Journal of virology · 2025Article
- Article
- Packaging defects in pestiviral NS4A can be compensated by mutations in NS2 and NS3.Journal of virology · 2023Article
- Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly.PLoS pathogens · 2022Article
- Identification of NS2 determinants stimulating intrinsic HCV NS2 protease activity.PLoS pathogens · 2022Article
- Membrane Topology of Pestiviral Non-Structural Protein 2 and determination of the minimal autoprotease domain.Journal of virology · 2021Article
- Review
- Molecular Events Occurring in Lipophagy and Its Regulation inFrontiers in microbiology · 2021Review
- Hijacking of Lipid Droplets by Hepatitis C, Dengue and Zika Viruses-From Viral Protein Moonlighting to Extracellular Release.International journal of molecular sciences · 2020Review
- The hypothetical role of phosphatidic acid in subverting ER membranes during SARS-CoV infection.Traffic (Copenhagen, Denmark) · 2020Article
- Determination of Critical Requirements for Classical Swine Fever Virus NS2-3-Independent Virion Formation.Journal of virology · 2019Article
- CRISPR/Cas9-Mediated Knockout of DNAJC14 Verifies This Chaperone as a Pivotal Host Factor for RNA Replication of Pestiviruses.Journal of virology · 2019Article
- Characterization of Recombinant Flaviviridae Viruses Possessing a Small Reporter Tag.Journal of virology · 2018Article
- High Abundance and Genetic Variability of Atypical Porcine Pestivirus in Pigs from Europe and Asia.Emerging infectious diseases · 2017Article
- Lipid droplet functions beyond energy storage.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- A positive-strand RNA virus uses alternative protein-protein interactions within a viral protease/cofactor complex to switch between RNA replication and virion morphogenesis.PLoS pathogens · 2017Article
- Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence.Veterinary research · 2017Article
- Characterization of the Determinants of NS2-3-Independent Virion Morphogenesis of Pestiviruses.Journal of virology · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nonstructural protein 5A (NS5A) of bovine viral diarrhea virus (BVDV) is a hydrophilic phosphoprotein with RNA binding activity and a critical component of the viral replicase. In silico analysis suggests that NS5A encompasses three domains interconnected by two low-complexity sequences (LCSs). While domain I harbors two functional determinants, an N-terminal amphipathic helix important for membrane association, and a Zn-binding site essential for RNA replication, the structure and function of the C-terminal half of NS5A are still ill defined. In this study, we introduced a panel of 10 amino acid deletions covering the C-terminal half of NS5A. In the context of a highly efficient monocistronic replicon, deletions in LCS I and the N-terminal part of domain II, as well as in domain III, were tolerated with regard to RNA replication. When introduced into a bicistronic replicon, only deletions in LCS I and the N-terminal part of domain II were tolerated. In the context of the viral full-length genome, these mutations allowed residual virion morphogenesis. Based on these data, a functional monocistronic BVDV replicon coding for an NS5A variant with an insertion of the fluorescent protein mCherry was constructed. Live cell imaging demonstrated that a fraction of NS5A-mCherry localizes to the surface of lipid droplets. Taken together, this study provides novel insights into the functions of BVDV NS5A. Moreover, we established the first pestiviral replicon expressing fluorescent NS5A-mCherry to directly visualize functional viral replication complexes by live cell imaging.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.