ArticleJournal of clinical microbiology2014
Development and application of a multiplex PCR method for rapid differential detection of subgroup A, B, and J avian leukosis viruses.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Detection of Avian Leukosis Virus Subgroup J (ALV-J) Using RAA and CRISPR-Cas13a Combined with Fluorescence and Lateral Flow Assay.International journal of molecular sciences · 2024Article
- Article
- Molecular Characteristics of Chicken Infectious Anemia Virus in Central and Eastern China from 2020 to 2022.Animals : an open access journal from MDPI · 2023Article
- Article
- Simultaneous and rapid detection of avian respiratory diseases of small poultry using multiplex reverse transcription-Polymerase Chain Reaction assay.Poultry science · 2023Article
- A Multiplex PCR Method for Simultaneous Detection of Infectious Laryngotracheitis Virus andVeterinary sciences · 2023Article
- Development and application of a reverse-transcription recombinase-aided amplification assay for subgroup J Avian leukosis virus.Poultry science · 2022Article
- Isolation and characterization of avian leukosis virus subgroup J associated with hemangioma and myelocytoma in layer chickens in China.Frontiers in veterinary science · 2022Article
- New rapid detection by using a constant temperature method for avian leukosis viruses.Frontiers in microbiology · 2022Article
- Establishment and Application of a Real-Time Recombinase Polymerase Amplification Assay for the Detection of Avian Leukosis Virus Subgroup J.Frontiers in veterinary science · 2022Article
- Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus.BMC veterinary research · 2020Article
- Co-infection with avian hepatitis E virus and avian leukosis virus subgroup J as the cause of an outbreak of hepatitis and liver hemorrhagic syndromes in a brown layer chicken flock in China.Poultry science · 2020Article
- Rapid detection of avian leukosis virus using a fluorescent microsphere immunochromatographic test strip assay.Poultry science · 2019Article
- Detection of ALV p27 in cloacal swabs and virus isolation medium by sELISA.BMC veterinary research · 2019Article
- Identification and characterization of a novel natural recombinant avian leucosis virus from Chinese indigenous chicken flock.Virus genes · 2019Article
- Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks.BMC veterinary research · 2019Article
- Molecular epidemiology of chicken anaemia virus in sick chickens in China from 2014 to 2015.PloS one · 2019Article
- An electrochemical immunosensor based on an etched zeolitic imidazolate framework for detection of avian leukosis virus subgroup J.Mikrochimica acta · 2018Article
- A multiplex PCR method for the simultaneous detection of three viruses associated with canine viral enteric infections.Archives of virology · 2018Article
- Development and application of a SYBR green real-time PCR for detection of the emerging avian leukosis virus subgroup K.Poultry science · 2018Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Avian leukosis virus (ALV) subgroups A, B, and J are very common in poultry flocks and have caused serious economic losses in recent years. A multiplex PCR (mPCR) method for the detection of these three subgroups was developed and optimized in this study. We first designed a common forward primer, PF, and three downstream primers, AR, BR, and JR, which can amplify 715 bp for subgroup A, 515 bp for subgroup B, and 422 bp for subgroup J simultaneously in one reaction. The mPCR method produced neither cross-reactions with other subgroups of ALVs nor nonspecific reactions with other common avian viruses. The detection limit of the mPCR was as low as 1 × 10(3) viral DNA copies of each of the three subgroups. In animal experiments, the mPCR detected ALVs 2 to 4 days earlier than did virus isolation from whole-blood samples and cloaca swabs. Furthermore, a total of 346 clinical samples (including 127 tissue samples, 86 cloaca swabs, 59 albumen samples, and 74 whole-blood samples) from poultry flocks with suspected ALV infection were examined by mPCR, routine PCR, and virus isolation. The positive sample/total sample ratios for ALV-A, ALV-B, and ALV-J were 48% (166/346) as detected by mPCR and 48% (166/346) as detected by routine PCR. However, the positive sample/total sample ratio detected by virus isolation was 40% (138/346). The results of the mPCR and routine PCR were confirmed by sequencing the specific fragments. These results indicate that the mPCR method is rapid, specific, sensitive, and convenient for use in epidemiological studies of ALV, clinical detection of ALV, and ALV eradication programs.
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