ArticleAnalytical chemistry2013
TOF-SIMS 3D imaging of native and non-native species within HeLa cells.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Molecular imaging of bacterial biofilms-a systematic review.Critical reviews in microbiology · 2024Pooled it
- Multi-modal single-cell platform for nanoparticle-enhanced time-series metabolic profiles of CD8Journal of nanobiotechnology · 2025Article
- Applications of Nanotechnology for Spatial Omics: Biological Structures and Functions at Nanoscale Resolution.ACS nano · 2025Review
- Nanokit coupled electrospray ionization mass spectrometry for analysis of angiotensin converting enzyme 2 activity in single living cell.Chinese chemical letters = Zhongguo hua xue kuai bao · 2023Article
- Advancements in ToF-SIMS imaging for life sciences.Frontiers in chemistry · 2023Review
- Article
- Submicron 3-D mass spectrometry imaging reveals an asymmetric molecular distribution on chemotaxing cells.F1000Research · 2022Article
- Single cell imaging reveals cisplatin regulating interactions between transcription (co)factors and DNA.Chemical science · 2021Article
- Nanoscopic subcellular imaging enabled by ion beam tomography.Nature communications · 2021Article
- Spatially resolved 3D metabolomic profiling in tissues.Science advances · 2021Article
- Novel Secondary Ion Mass Spectrometry Methods for the Examination of Metabolic Effects at the Cellular and Subcellular Levels.Frontiers in behavioral neuroscience · 2020Article
- Exploring the Fundamental Structures of Life: Non-Targeted, Chemical Analysis of Single Cells and Subcellular Structures.Angewandte Chemie (International ed. in English) · 2019Review
- Correlated Materials Characterization via Multimodal Chemical and Functional Imaging.ACS nano · 2018Article
- Towards enhanced metabolomic data analysis of mass spectrometry image: Multivariate Curve Resolution and Machine Learning.Analytica chimica acta · 2018Article
- Analysis of the Myc-induced pancreaticBiointerphases · 2018Article
- Observation of endoplasmic reticulum tubules via TOF-SIMS tandem mass spectrometry imaging of transfected cells.Biointerphases · 2018Article
- Tip-enhanced ablation and ionization mass spectrometry for nanoscale chemical analysis.Science advances · 2017Article
- Biomedical surface analysis: Evolution and future directions (Review).Biointerphases · 2017Review
- Nanoscale physicochemical properties of chain- and step-growth polymerized PEG hydrogels affect cell-material interactions.Journal of biomedical materials research. Part A · 2017Article
- Near-Atomic Three-Dimensional Mapping for Site-Specific Chemistry of 'Superbugs'.Nano letters · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In this study, a non-native chemical species, bromodeoxyuridine (BrdU), was imaged within single HeLa cells using time-of-flight secondary ion mass spectrometry (TOF-SIMS). z-corrected 3D images were reconstructed that accurately portray the distribution of intracellular BrdU as well as other intracellular structures. The BrdU was localized to the nucleus of cells, whereas structures composed of CxHyOz(-) species were located in bundles on the periphery of cells. The CxHyOz(-) subcellular features had a spatial resolution at or slightly below a micrometer (900 nm), as defined by the distance between the 16% and 84% intensities in a line scan across the edge of the features. Additionally, important parameters influencing the quality of the HeLa cell 3D images were investigated. Atomic force microscopy measurements revealed that the HeLa cells were sputtered at a rate of approximately 4 nm per 10(13) C60(+) ions/cm(2) at 10 keV and a 45° incident angle. Optimal 3D images were acquired using a Bi3(+) liquid metal ion gun operating in the simultaneous high mass and spatial resolution mode.
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Registered trials
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