ArticleSpringerPlus2013
Molecular cloning and development of RAPD-SCAR markers for Dimocarpus longan variety authentication.
Article in SpringerPlus, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Development of One-Tube Multiplex Arbitrary (RAPD and ISSR) Marker-Based SCAR Assay for Simultaneous Detection and Authentication of Indian Senna (International journal of molecular sciences · 2026Article
- Correlation between molecular diversity and biochemical traits of edible aerial parts of Basella alba L. from different geographical locations of Sri Lanka.BMC genomic data · 2025Article
- In Vitro Culture Technology and Advanced Biotechnology Tools Toward Improvement in Gladiolus (Gladiolus species): Present Scenario and Future Prospects.Molecular biotechnology · 2024Review
- Development of highly discriminatory SCoT- and CBDP-based SCAR fingerprint for authentication of Indian senna (Frontiers in plant science · 2024Article
- Genetic Analysis ofGenetics research · 2022Article
- Random Amplified Polymorphic DNA (RAPD) and Derived Techniques.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Climate-Resilient Dairy Cattle Production: Applications of Genomic Tools and Statistical Models.Frontiers in veterinary science · 2021Review
- RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae).Molecular biology reports · 2020Article
- Article
- Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. strains into grapevine plants.PloS one · 2019Article
- Genotyping and Bio-Sensing Chemosensory Proteins in Insects.Sensors (Basel, Switzerland) · 2017Review
- A First Phylogeny of the Genus Dimocarpus and Suggestions for Revision of Some Taxa Based on Molecular and Morphological Evidence.Scientific reports · 2017Article
- Use of Moringa oleifera Flower Pod Extract as Natural Preservative and Development of SCAR Marker for Its DNA Based Identification.BioMed research international · 2016Article
- Article
- Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers.Molecules (Basel, Switzerland) · 2015Article
- Developing single nucleotide polymorphism (SNP) markers from transcriptome sequences for identification of longan (Dimocarpus longan) germplasm.Horticulture research · 2015Article
- Genetic analysis of litchi (Litchi chinensis Sonn.) in southern China by improved random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR).Molecular biology reports · 2015Article
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5 authors.
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Abstract
As an edible fruit and source of traditional medicine, D. longan is grown in most areas of Southern China. Identification of D. longan cultivars by using molecular markers is important genetically. In this study, we cloned fragments from improved randomly amplified polymorphic DNA (RAPD), and developed stably diagnostic sequence-characterized amplified region (SCAR) markers. The specific RAPD bands of D. longan cultivars from Guangxi, with size ranging from 500 bp to 900 bp were gel-purified, cloned and sequenced. Four clones named LY2-1, LY4-7, LY4-8 and LY5-2 were identified. In order to investigate whether the fragments were specific for the species, four pairs of SCAR primers were then designed. PCR amplifications were conducted to analyze 18 samples including different D. longan cultivars and other species. The specific bands with expected sizes were amplified in five D. longan samples but not in others. To identify and characterize the difference between D. longan and D. confinis, PCR amplifications were performed again. The specific bands with expected sizes were found in D. longan but not in D. confinis by SCAR markers LY2-1, LY4-7 and LY5-2, respectively. These results showed that our developed SCAR markers could be very useful as a specific D. longan variety authentication. Therefore, our study provides an effective and precise PCR-based diagnostic method and markers to identify D. longan species.
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