ArticlePloS one2013
Molecular characterization of the α-subunit of Na⁺/K⁺ ATPase from the euryhaline barnacle Balanus improvisus reveals multiple genes and differential expression of alternative splice variants.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Ten years of marine evolutionary biology-Challenges and achievements of a multidisciplinary research initiative.Evolutionary applications · 2023Article
- Salinity significantly affects intestinal microbiota and gene expression in striped catfish juveniles.Applied microbiology and biotechnology · 2022Article
- Genomic Characterization of the Barnacle Balanus improvisus Reveals Extreme Nucleotide Diversity in Coding Regions.Marine biotechnology (New York, N.Y.) · 2021Article
- Physiological basis of interactive responses to temperature and salinity in coastal marine invertebrate: Implications for responses to warming.Ecology and evolution · 2021Article
- NaThe Journal of membrane biology · 2020Review
- Dilution of Seawater Affects the CaFrontiers in physiology · 2020Article
- Osmoregulation in Barnacles: An Evolutionary Perspective of Potential Mechanisms and Future Research Directions.Frontiers in physiology · 2019Review
- Article
- Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae.Scientific reports · 2018Article
- The Barnacle Balanus improvisus as a Marine Model - Culturing and Gene Expression.Journal of visualized experiments : JoVE · 2018Article
- Article
- Importance of plasticity and local adaptation for coping with changing salinity in coastal areas: a test case with barnacles in the Baltic Sea.BMC evolutionary biology · 2014Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The euryhaline bay barnacle Balanus improvisus has one of the broadest salinity tolerances of any barnacle species. It is able to complete its life cycle in salinities close to freshwater (3 PSU) up to fully marine conditions (35 PSU) and is regarded as one of few truly brackish-water species. Na⁺/K⁺ ATPase (NAK) has been shown to be important for osmoregulation when marine organisms are challenged by changing salinities, and we therefore cloned and examined the expression of different NAKs from B. improvisus. We found two main gene variants, NAK1 and NAK2, which were approximately 70% identical at the protein level. The NAK1 mRNA existed in a long and short variant with the encoded proteins differing only by 27 N-terminal amino acids. This N-terminal stretch was coded for by a separate exon, and the two variants of NAK1 mRNAs appeared to be created by alternative splicing. We furthermore showed that the two NAK1 isoforms were differentially expressed in different life stages and in various tissues of adult barnacle, i.e the long isoform was predominant in cyprids and in adult cirri. In barnacle cyprid larvae that were exposed to a combination of different salinities and pCO2 levels, the expression of the long NAK1 mRNA increased relative to the short in low salinities. We suggest that the alternatively spliced long variant of the Nak1 protein might be of importance for osmoregulation in B. improvisus in low salinity conditions.
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