ArticlePloS one2013
Inheritable and precise large genomic deletions of non-coding RNA genes in zebrafish using TALENs.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin.Science (New York, N.Y.) · 2023Article
- Phosphoproteomics Reveals the AMPK Substrate Network in Response to DNA Damage and Histone Acetylation.Genomics, proteomics & bioinformatics · 2022Article
- Ythdf mCell reports · 2020Article
- Genetic Deletion ofFrontiers in genetics · 2020Article
- Brd4 and P300 Confer Transcriptional Competency during Zygotic Genome Activation.Developmental cell · 2019Article
- High Efficiency Targeting of Non-coding Sequences Using CRISPR/Cas9 System in Tilapia.G3 (Bethesda, Md.) · 2019Article
- Transcriptomic Analysis of Potential "lncRNA-mRNA" Interactions in Liver of the Marine TeleostFrontiers in physiology · 2019Article
- Analyses of mRNA structure dynamics identify embryonic gene regulatory programs.Nature structural & molecular biology · 2018Article
- Long Noncoding RNA Discovery in Cardiovascular Disease: Decoding Form to Function.Circulation research · 2018Review
- Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (International journal of biological sciences · 2018Article
- Fertility Enhancement but Premature Ovarian Failure inFrontiers in endocrinology · 2018Article
- Spexin Suppress Food Intake in Zebrafish: Evidence from Gene Knockout Study.Scientific reports · 2017Article
- Germline-specific dgcr8 knockout in zebrafish using a BACK approach.Cellular and molecular life sciences : CMLS · 2017Article
- When Long Noncoding RNAs Meet Genome Editing in Pluripotent Stem Cells.Stem cells international · 2017Review
- Animal Models to Study MicroRNA Function.Advances in cancer research · 2017Review
- In vivo mutagenesis of miRNA gene families using a scalable multiplexed CRISPR/Cas9 nuclease system.Scientific reports · 2016Article
- Investigating long noncoding RNAs using animal models.The Journal of clinical investigation · 2016Review
- Gene knockout of nuclear progesterone receptor provides insights into the regulation of ovulation by LH signaling in zebrafish.Scientific reports · 2016Article
- Gonadotropin Signaling in Zebrafish Ovary and Testis Development: Insights From Gene Knockout Study.Molecular endocrinology (Baltimore, Md.) · 2015Article
- Direct production of XY(DMY-) sex reversal female medaka (Oryzias latipes) by embryo microinjection of TALENs.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription activator-like effector nucleases (TALENs) have so far been applied to disrupt protein-coding genes which constitute only 2-3% of the genome in animals. The majority (70-90%) of the animal genome is actually transcribed as non-coding RNAs (ncRNAs), yet the lack of efficient tools to knockout ncRNA genes hinders studies on their in vivo functions. Here we have developed novel strategies using TALENs to achieve precise and inheritable large genomic deletions and knockout of ncRNA genes in zebrafish. We have demonstrated that individual miRNA genes could be disrupted using one pair of TALENs, whereas large microRNA (miRNA) gene clusters and long non-coding RNA (lncRNA) genes could be precisely deleted using two pairs of TALENs. We have generated large genomic deletions of two miRNA clusters (the 1.2 kb miR-17-92 cluster and the 79.8 kb miR-430 cluster) and one long non-coding RNA (lncRNA) gene (the 9.0 kb malat1), and the deletions are transmitted through the germline. Taken together, our results establish TALENs as a robust tool to engineer large genomic deletions and knockout of ncRNA genes, thus opening up new avenues in the application of TALENs to study the genome in vivo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.