ArticlePloS one2013
Efficient and rapid C. elegans transgenesis by bombardment and hygromycin B selection.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Targeted integration of extrachromosomal arrays inScience advances · 2026Article
- Photocaged FLP recombinase for precise spatio-temporal control of gene expression.Nucleic acids research · 2026Article
- Impairment of neuronal activity occurs at the early stages of the aggregation cascade of Aβ1-42 and mutant Tau.Disease models & mechanisms · 2026Article
- Notch activity is modulated by the aGPCR Latrophilin binding the DSL ligand in C. elegans.Nature communications · 2025Article
- Intraspecific variation in the duration of epigenetic inheritance.bioRxiv : the preprint server for biology · 2025Article
- Screening conditions and constructs for attempted genetic transformation of C. elegans by Agrobacterium.PloS one · 2025Article
- Microparticle Bombardment as a Method for Transgenesis inmicroPublication biology · 2025Article
- The N terminus-only (trans) function of the adhesion G protein-coupled receptor latrophilin-1 controls multiple processes in reproduction of Caenorhabditis elegans.G3 (Bethesda, Md.) · 2024Article
- Characterizing a standardized BioPart for BAG-specific expression inmicroPublication biology · 2024Article
- Electric shock causes a fleeing-like persistent behavioral response in the nematode Caenorhabditis elegans.Genetics · 2023Article
- High-throughput library transgenesis ineLife · 2023Article
- FLInt: single shot safe harbor transgene integration via Fluorescent Landmark Interference.G3 (Bethesda, Md.) · 2023Article
- Characterizing a standardized BioPart for PVQ-specific expression inmicroPublication biology · 2023Article
- Clinical variants inGenetics in medicine open · 2023Article
- Highly efficient transgenesis with miniMos in Caenorhabditis briggsae.G3 (Bethesda, Md.) · 2022Article
- Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2022Article
- Using a quadruplet codon to expand the genetic code of an animal.Nucleic acids research · 2022Article
- NanoBRET in C. elegans illuminates functional receptor interactions in real time.BMC molecular and cell biology · 2022Article
- Integration of multicopy extrachromosomal transgenes into defined loci without phenotypes.microPublication biology · 2022Article
- Transgenesis of the gonochoristic nematodemicroPublication biology · 2022Article
Corrections and comments
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Authors and funding
3 authors.
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Abstract
We report a simple, cost-effective, scalable and efficient method for creating transgenic Caenorhabditis elegans that requires minimal hands-on time. The method combines biolistic bombardment with selection for transgenics that bear a hygromycin B resistance gene on agar plates supplemented with hygromycin B, taking advantage of our observation that hygromycin B is sufficient to kill wild-type C. elegans at very low concentrations. Crucially, the method provides substantial improvements in the success of bombardments for isolating transmitting strains, the isolation of multiple independent strains, and the isolation of integrated strains: 100% of bombardments in a large data set yielded transgenics; 10 or more independent strains were isolated from 84% of bombardments, and up to 28 independent strains were isolated from a single bombardment; 82% of bombardments yielded stably transmitting integrated lines with most yielding multiple integrated lines. We anticipate that the selection will be widely adopted for C. elegans transgenesis via bombardment, and that hygromycin B resistance will be adopted as a marker in other approaches for manipulating, introducing or deleting DNA in C. elegans.
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