ArticleFrontiers in neuroanatomy2013
Intravital imaging of hair-cell development and regeneration in the zebrafish.
Article in Frontiers in neuroanatomy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Quantitative videomicroscopy reveals latent control of cell-pair rotations in vivo.Development (Cambridge, England) · 2023Article
- Neuromasts and Olfactory Organs of Zebrafish Larvae Represent Possible Sites of SARS-CoV-2 Pseudovirus Host Cell Entry.Journal of virology · 2022Article
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Functional development and regeneration of hair cells in the zebrafish lateral line.The Journal of physiology · 2021Article
- Zebrafish as a Model for Fish Diseases in Aquaculture.Pathogens (Basel, Switzerland) · 2020Review
- Article
- Behavioral methods for the functional assessment of hair cells in zebrafish.Frontiers of medicine · 2017Review
- Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses.Neural plasticity · 2017Article
- Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development.Journal of visualized experiments : JoVE · 2016Article
- Inexhaustible hair-cell regeneration in young and aged zebrafish.Biology open · 2015Article
- Sensory hair cell death and regeneration in fishes.Frontiers in cellular neuroscience · 2015Review
- Article
- Neuroanatomy and transgenic technologies.Frontiers in neuroanatomy · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct videomicroscopic visualization of organ formation and regeneration in toto is a powerful strategy to study cellular processes that often cannot be replicated in vitro. Intravital imaging aims at quantifying changes in tissue architecture or subcellular organization over time during organ development, regeneration or degeneration. A general feature of this approach is its reliance on the optical isolation of defined cell types in the whole animals by transgenic expression of fluorescent markers. Here we describe a simple and robust method to analyze sensory hair-cell development and regeneration in the zebrafish lateral line by high-resolution intravital imaging using laser-scanning confocal microscopy (LSCM) and selective plane illumination microscopy (SPIM). The main advantage of studying hair-cell regeneration in the lateral line is that it occurs throughout the life of the animal, which allows its study in the most natural context. We detail protocols to achieve continuous videomicroscopy for up to 68 hours, enabling direct observation of cellular behavior, which can provide a sensitive assay for the quantitative classification of cellular phenotypes and cell-lineage reconstruction. Modifications to this protocol should facilitate pharmacogenetic assays to identify or validate otoprotective or reparative drugs for future clinical strategies aimed at preserving aural function in humans.
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