ArticleThe Plant cell2013
Plant purine nucleoside catabolism employs a guanosine deaminase required for the generation of xanthosine in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 55 citations in OpenAlex.
- A plastid carbohydrate carrier mediates ribose recycling from nucleotide catabolism and glucose export from starch degradation.Nature communications · 2025Article
- Single-Cell Transcriptome Atlas and Dynamic Regulatory Mechanisms of Anther Development in Alfalfa (Medicago sativa L.).Plant biotechnology journal · 2025Article
- Seed Metabolomic Landscape Reflecting Key Differential Metabolic Profiles Among Different Wheat Cultivars.Metabolites · 2025Article
- Three Arabidopsis UMP kinases have different roles in pyrimidine nucleotide biosynthesis and (deoxy)CMP salvage.The Plant cell · 2024Article
- HNCGAT: a method for predicting plant metabolite-protein interaction using heterogeneous neighbor contrastive graph attention network.Briefings in bioinformatics · 2024Article
- Discovery of a novel nucleoside immune signaling molecule 2'-deoxyguanosine in microbes and plants.Journal of advanced research · 2023Article
- New cup out of old coffee: contribution of parental gene expression legacy to phenotypic novelty in coffee beans of the allopolyploid Coffea arabica L.Annals of botany · 2023Article
- A plastid nucleoside kinase is involved in inosine salvage and control of purine nucleotide biosynthesis.The Plant cell · 2023Article
- Binding asymmetry and conformational studies of the AtGSDA dimer.Computational and structural biotechnology journal · 2023Article
- Transcriptional reprogramming of nucleotide metabolism in response to altered pyrimidine availability in Arabidopsis seedlings.Frontiers in plant science · 2023Article
- Substrate Specificity of GSDA Revealed by Cocrystal Structures and Binding Studies.International journal of molecular sciences · 2022Article
- The nucleotide metabolome of germinating Arabidopsis thaliana seeds reveals a central role for thymidine phosphorylation in chloroplast development.The Plant cell · 2022Article
- Enzymes and cellular interplay required for flux of fixed nitrogen to ureides in bean nodules.Nature communications · 2022Article
- Purification and Analysis of Nucleotides and Nucleosides from Plants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Novel Guanine Elicitor Stimulates Immunity inFrontiers in plant science · 2022Article
- Initiation of cytosolic plant purine nucleotide catabolism involves a monospecific xanthosine monophosphate phosphatase.Nature communications · 2021Article
- A drought-responsive rice amidohydrolase is the elusive plant guanine deaminase with the potential to modulate the epigenome.Physiologia plantarum · 2021Article
- Enhanced nucleotide analysis enables the quantification of deoxynucleotides in plants and algae revealing connections between nucleoside and deoxynucleoside metabolism.The Plant cell · 2021Article
- Analysis of Nucleosides and Nucleotides in Plants: An Update on Sample Preparation and LC-MS Techniques.Cells · 2021Review
- Nucleoside Metabolism Is Induced in Common Bean During Early Seedling Development.Frontiers in plant science · 2021Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Purine nucleotide catabolism is common to most organisms and involves a guanine deaminase to convert guanine to xanthine in animals, invertebrates, and microorganisms. Using metabolomic analysis of mutants, we demonstrate that Arabidopsis thaliana uses an alternative catabolic route employing a highly specific guanosine deaminase (GSDA) not reported from any organism so far. The enzyme is ubiquitously expressed and deaminates exclusively guanosine and 2'-deoxyguanosine but no other aminated purines, pyrimidines, or pterines. GSDA belongs to the cytidine/deoxycytidylate deaminase family of proteins together with a deaminase involved in riboflavin biosynthesis, the chloroplastic tRNA adenosine deaminase Arg and a predicted tRNA-specific adenosine deaminase 2 in A. thaliana. GSDA is conserved in plants, including the moss Physcomitrella patens, but is absent in the algae and outside the plant kingdom. Our data show that xanthosine is exclusively generated through the deamination of guanosine by GSDA in A. thaliana, excluding other possible sources like the dephosphorylation of xanthosine monophosphate. Like the nucleoside hydrolases NUCLEOSIDE HYDROLASE1 (NSH1) and NSH2, GSDA is located in the cytosol, indicating that GMP catabolism to xanthine proceeds in a mostly cytosolic pathway via guanosine and xanthosine. Possible implications for the biosynthetic route of purine alkaloids (caffeine and theobromine) and ureides in other plants are discussed.
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