ArticleApoptosis : an international journal on programmed cell death2014
Apoptosis in differentiating C2C12 muscle cells selectively targets Bcl-2-deficient myotubes.
Article in Apoptosis : an international journal on programmed cell death, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 60 citations in OpenAlex.
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- An atlas of caspase cleavage events in differentiating muscle cells.Protein science : a publication of the Protein Society · 2024Article
- Anti-apoptotic protein Bcl-2 contributes to the determination of reserve cells during myogenic differentiation of C2C12 cells.In vitro cellular & developmental biology. Animal · 2024Article
- Cellular Effects ofInternational journal of molecular sciences · 2024Article
- FoxP1 Represses MEF2A in Striated Muscle.Molecular and cellular biology · 2024Article
- Indian Ornamental Tarantula (Cells · 2023Article
- Exosome-mediated regulatory mechanisms in skeletal muscle: a narrative review.Journal of Zhejiang University. Science. B · 2023Review
- Homer 1 genotype AA variant relates to congenital splay leg syndrome in piglets by repressing Pax7 in myogenic progenitors.Frontiers in veterinary science · 2023Article
- Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy underSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2022Article
- Identification of Robust and Key Differentially Expressed Genes during C2C12 Cell Myogenesis Based on Multiomics Data.International journal of molecular sciences · 2022Article
- Sepsis induces muscle atrophy by inhibiting proliferation and promoting apoptosis via PLK1-AKT signalling.Journal of cellular and molecular medicine · 2021Article
- Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes.Science advances · 2021Article
- Myomerger promotes fusion pore by elastic coupling between proximal membrane leaflets and hemifusion diaphragm.Nature communications · 2021Article
- Kinetin stimulates differentiation of C2C12 myoblasts.PloS one · 2021Article
- SMaRT lncRNA controls translation of a G-quadruplex-containing mRNA antagonizing the DHX36 helicase.EMBO reports · 2020Article
- Zfp422 promotes skeletal muscle differentiation by regulating EphA7 to induce appropriate myoblast apoptosis.Cell death and differentiation · 2020Article
- Article
- Differential responses of myoblasts and myotubes to photobiomodulation are associated with mitochondrial number.Journal of biophotonics · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Muscle cell apoptosis accompanies normal muscle development and regeneration, as well as degenerative diseases and aging. C2C12 murine myoblast cells represent a common model to study muscle differentiation. Though it was already shown that myogenic differentiation of C2C12 cells is accompanied by enhanced apoptosis in a fraction of cells, either the cell population sensitive to apoptosis or regulatory mechanisms for the apoptotic response are unclear so far. In the current study we characterize apoptotic phenotypes of different types of C2C12 cells at all stages of differentiation, and report here that myotubes of differentiated C2C12 cells with low levels of anti-apoptotic Bcl-2 expression are particularly vulnerable to apoptosis even though they are displaying low levels of pro-apoptotic proteins Bax, Bak and Bad. In contrast, reserve cells exhibit higher levels of Bcl-2 and high resistance to apoptosis. The transfection of proliferating myoblasts with Bcl-2 prior to differentiation did not protect against spontaneous apoptosis accompanying differentiation of C2C12 cells but led to Bcl-2 overexpression in myotubes and to significant protection from apoptotic cell loss caused by exposure to hydrogen peroxide. Overall, our data advocate for a Bcl-2-dependent mechanism of apoptosis in differentiated muscle cells. However, downstream processes for spontaneous and hydrogen peroxide induced apoptosis are not completely similar. Apoptosis in differentiating myoblasts and myotubes is regulated not through interaction of Bcl-2 with pro-apoptotic Bcl-2 family proteins such as Bax, Bak, and Bad.
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