ArticleThe Journal of biological chemistry2013
Structure of the Toxoplasma gondii ROP18 kinase domain reveals a second ligand binding pocket required for acute virulence.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Therapeutic Antitoxoplasmosis Potential ofJournal of tropical medicine · 2026Article
- Cysteine-S-nitrosylation inhibits Rop5-mediated immune evasion inbioRxiv : the preprint server for biology · 2025Article
- Proteomic approaches for protein kinase substrate identification in Apicomplexa.Molecular and biochemical parasitology · 2024Review
- Unravelling Strain-Specific Modifications of Toxoplasma gondii tRNA and sncRNA Using LC-MS/MS.Microbiology spectrum · 2023Article
- ROP39 is an Irgb10-specific parasite effector that modulates acute Toxoplasma gondii virulence.PLoS pathogens · 2023Article
- Redox priming promotes Aurora A activation during mitosis.Science signaling · 2020Article
- Prediction of Toxoplasma gondii virulence factor ROP18 competitive inhibitors by virtual screening.Parasites & vectors · 2019Article
- Protein targets of thiazolidinone derivatives in Toxoplasma gondii and insights into their binding to ROP18.BMC genomics · 2018Article
- A Human Proteome Array Approach to Identifying Key Host Proteins Targeted byMolecular & cellular proteomics : MCP · 2017Article
- Interferon-Inducible GTPases in Host Resistance, Inflammation and Disease.Journal of molecular biology · 2016Review
- Article
- Identification of small molecule inhibitors that block theACS infectious diseases · 2016Article
- Neurons are the Primary Target Cell for the Brain-Tropic Intracellular Parasite Toxoplasma gondii.PLoS pathogens · 2016Article
- The Toxoplasma gondii rhoptry protein ROP18 is an Irga6-specific kinase and regulated by the dense granule protein GRA7.Cellular microbiology · 2016Article
- Enzyme Activity Assays for Protein Kinases: Strategies to Identify Active Substrates.Current drug discovery technologies · 2016Review
- Biochemical characterization of FIKK8--A unique protein kinase from the malaria parasite Plasmodium falciparum and other apicomplexans.Molecular and biochemical parasitology · 2015Article
- Protective efficacy of recombinant canine adenovirus type-2 expressing TgROP18 (CAV-2-ROP18) against acute and chronic Toxoplasma gondii infection in mice.BMC infectious diseases · 2015Article
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
At least a third of the human population is infected with the intracellular parasite Toxoplasma gondii, which contributes significantly to the disease burden in immunocompromised and neutropenic hosts and causes serious congenital complications when vertically transmitted to the fetus. Genetic analyses have identified the Toxoplasma ROP18 Ser/Thr protein kinase as a major factor mediating acute virulence in mice. ROP18 is secreted into the host cell during the invasion process, and its catalytic activity is required for the acute virulence phenotype. However, its precise molecular function and regulation are not fully understood. We have determined the crystal structure of the ROP18 kinase domain, which is inconsistent with a previously proposed autoinhibitory mechanism of regulation. Furthermore, a sucrose molecule bound to our structure identifies an additional ligand-binding pocket outside of the active site cleft. Mutational analysis confirms an important role for this pocket in virulence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.