ArticleRNA (New York, N.Y.)2013
Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus.
Article in RNA (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Introduction of the Ribo-BiFC method to plants using a split mVenus approach.Plant methods · 2026Article
- The nuclear cap-binding complex regulates subcellular RNA processing and surveillance of coding and noncoding RNAs.Nucleic acids research · 2026Article
- Experimental approaches to studying translation in plant semi-autonomous organelles.Journal of experimental botany · 2024Review
- UPF3B modulates endoplasmic reticulum stress through interaction with inositol-requiring enzyme-1α.Cell death & disease · 2024Article
- Has translation in the nucleus found its purpose?Nature reviews. Molecular cell biology · 2024Article
- Enhanced nuclear translation is associated with proliferation and progression across multiple cancers.MedComm · 2023Article
- mtIF3 is locally translated in axons and regulates mitochondrial translation for axonal growth.BMC biology · 2022Article
- Localization and Functional Roles of Components of the Translation Apparatus in the Eukaryotic Cell Nucleus.Cells · 2021Review
- Evidence in disease and non-disease contexts that nonsense mutations cause altered splicing via motif disruption.Nucleic acids research · 2021Article
- Local translation in nuclear condensate amyloid bodies.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- mRNA Targeting, Transport and Local Translation in Eukaryotic Cells: From the Classical View to a Diversity of New Concepts.Molecular biology · 2021Article
- Visualisation of ribosomes inBiology open · 2020Article
- The science of puromycin: From studies of ribosome function to applications in biotechnology.Computational and structural biotechnology journal · 2020Review
- An mRNA-binding channel in the ES6S region of the translation 48S-PIC promotes RNA unwinding and scanning.eLife · 2019Article
- Manganese-induced cellular disturbance in the baker's yeast, Saccharomyces cerevisiae with putative implications in neuronal dysfunction.Scientific reports · 2019Article
- Nuclear processing of nascent transcripts determines synthesis of full-length proteins and antigenic peptides.Nucleic acids research · 2019Article
- Flu DRiPs in MHC Class I Immunosurveillance.Virologica Sinica · 2019Review
- Article
- Chromatin capture links the metabolic enzyme AHCY to stem cell proliferation.Science advances · 2019Article
- Exon junction complex proteins bind nascent transcripts independently of pre-mRNA splicing ineLife · 2016Article
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Authors and funding
11 authors.
Funding
Abstract
In eukaryotes the 40S and 60S ribosomal subunits are assembled in the nucleolus, but there appear to be mechanisms preventing mRNA binding, 80S formation, and initiation of translation in the nucleus. To visualize association between ribosomal subunits, we tagged pairs of Drosophila ribosomal proteins (RPs) located in different subunits with mutually complementing halves of fluorescent proteins. Pairs of tagged RPs expected to interact, or be adjacent in the 80S structure, showed strong fluorescence, while pairs that were not in close proximity did not. Moreover, the complementation signal is found in ribosomal fractions and it was enhanced by translation elongation inhibitors and reduced by initiation inhibitors. Our technique achieved 80S visualization both in cultured cells and in fly tissues in vivo. Notably, while the main 80S signal was in the cytoplasm, clear signals were also seen in the nucleolus and at other nuclear sites. Furthermore, we detected rapid puromycin incorporation in the nucleolus and at transcription sites, providing an independent indication of functional 80S in the nucleolus and 80S association with nascent transcripts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.