ArticleThe Journal of investigative dermatology2014
Real-time monitoring of oxidative stress in live mouse skin.
Article in The Journal of investigative dermatology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
- Label-free and real-time monitoring of photoaging with high spatiotemporal resolution using an nIR fluorescent nanosensor array.Science advances · 2025Article
- Promising tools into oxidative stress: A review of non-rodent model organisms.Redox biology · 2024Review
- Antioxidants in Photoaging: From Molecular Insights to Clinical Applications.International journal of molecular sciences · 2024Review
- Oxidative stress monitoring in iPSC-derived motor neurons using genetically encoded biosensors of HScientific reports · 2022Article
- Fluorescent protein transgenic mice for the study of CaFree radical biology & medicine · 2022Review
- BP180/Collagen XVII: A Molecular View.International journal of molecular sciences · 2021Review
- Ultraviolet A irradiation induces ultraweak photon emission with characteristic spectral patterns from biomolecules present in human skin.Scientific reports · 2020Article
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors.Journal of visualized experiments : JoVE · 2018Article
- ROS in Cancer: The Burning Question.Trends in molecular medicine · 2017Review
- Mitochondrially targeted fluorescent redox sensors.Interface focus · 2017Review
- Live-cell imaging approaches for the investigation of xenobiotic-induced oxidant stress.Biochimica et biophysica acta · 2016Review
- Redox Indicator Mice Stably Expressing Genetically Encoded Neuronal roGFP: Versatile Tools to Decipher Subcellular Redox Dynamics in Neuropathophysiology.Antioxidants & redox signaling · 2016Article
- Imaging Reactive Oxygen Species-Induced Modifications in Living Systems.Antioxidants & redox signaling · 2016Review
- Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures.Clinical interventions in aging · 2016Article
- Cells to surgery quiz: August 2014: reactive oxygen species mediate photoaging.The Journal of investigative dermatology · 2014Article
- Light-emitting hair follicles: studying skin regeneration with in vivo imaging.The Journal of investigative dermatology · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxidative stress is involved in many age-associated diseases, as well as in the aging process itself. The development of interventions to reduce oxidative stress is hampered by the absence of sensitive detection methods that can be used in live animals. We generated transgenic mice expressing ratiometric redox-sensitive green fluorescent protein (roGFP) in the cytosol or mitochondria of several tissues, including skin epidermal keratinocytes. Crossbreeding into hairless albino mice allowed noninvasive optical measurement of skin oxidative state. Topical application of hydrogen peroxide emulsion shifted the keratinocyte redox state toward oxidation within minutes and could be observed in real time by fluorescence ratio imaging. Exposing skin to 365 nm UVA radiation oxidized roGFP localized in keratinocyte mitochondria, but not when roGFP was localized in the cytosol. This suggests that significant amounts of the endogenous photosensitizers that mediate UVA-induced oxidative stress are located in the mitochondria. UVR is the major environmental cause of skin aging and UVA-mediated oxidative stress has been associated with the development of wrinkles in humans. Direct measurements of redox state in defined cell compartments of live animals should be a powerful and convenient tool for evaluating treatments that aim to modulate oxidative stress.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.