ArticleGenome biology and evolution2013
Evaluating the fitness cost of protein expression in Saccharomyces cerevisiae.
Article in Genome biology and evolution, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Fitness costs of mobilised colistin resistance gene 3 (mcr-3): systematic review, epidemiological study, and functional analysis.EBioMedicine · 2025Pooled it
- Determining frequencies of fungicide resistance in Leptosphaeria maculans that render demethylation inhibitor fungicides ineffective in the field.Pest management science · 2026Article
- Molecular evolution in light of regulatory-coding epistasis.EMBO reports · 2026Review
- Downregulation is the dominant effect of new regulatory mutations in a fungal pathogen.Microbial genomics · 2026Article
- Overexpression of a single ORF can extend chronological lifespan in yeast if retrograde signaling and stress response are stimulated.Biogerontology · 2021Article
- An Overexpression Experiment Does Not Support the Hypothesis That Avoidance of Toxicity Determines the Rate of Protein Evolution.Genome biology and evolution · 2020Article
- High Transcriptional Error Rates Vary as a Function of Gene Expression Level.Genome biology and evolution · 2020Article
- Protein Abundance Biases the Amino Acid Composition of Disordered Regions to Minimize Non-functional Interactions.Journal of molecular biology · 2019Article
- Light-based control of metabolic flux through assembly of synthetic organelles.Nature chemical biology · 2019Article
- Gene overexpression screen for chromosome instability in yeast primarily identifies cell cycle progression genes.Current genetics · 2019Article
- Measuring Single-Cell Phenotypic Growth Heterogeneity Using a Microfluidic Cell Volume Sensor.Scientific reports · 2018Article
- Stoichiometric balance of protein copy numbers is measurable and functionally significant in a protein-protein interaction network for yeast endocytosis.PLoS computational biology · 2018Article
- Stress Introduction Rate Alters the Benefit of AcrAB-TolC Efflux Pumps.Journal of bacteriology · 2018Article
- The effect of extrinsic mortality on genome size evolution in prokaryotes.The ISME journal · 2017Article
- Cellular growth defects triggered by an overload of protein localization processes.Scientific reports · 2016Article
- A Theoretical Lower Bound for Selection on the Expression Levels of Proteins.Genome biology and evolution · 2016Article
- Heterozygote Advantage Is a Common Outcome of Adaptation in Saccharomyces cerevisiae.Genetics · 2016Article
- Article
- Quantitative nature of overexpression experiments.Molecular biology of the cell · 2015Article
- Opposing effects of target overexpression reveal drug mechanisms.Nature communications · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein metabolism is one of the most costly processes in the cell and is therefore expected to be under the effective control of natural selection. We stimulated yeast strains to overexpress each single gene product to approximately 1% of the total protein content. Consistent with previous reports, we found that excessive expression of proteins containing disordered or membrane-protruding regions resulted in an especially high fitness cost. We estimated these costs to be nearly twice as high as for other proteins. There was a ten-fold difference in cost if, instead of entire proteins, only the disordered or membrane-embedded regions were compared with other segments. Although the cost of processing bulk protein was measurable, it could not be explained by several tested protein features, including those linked to translational efficiency or intensity of physical interactions after maturation. It most likely included a number of individually indiscernible effects arising during protein synthesis, maturation, maintenance, (mal)functioning, and disposal. When scaled to the levels normally achieved by proteins in the cell, the fitness cost of dealing with one amino acid in a standard protein appears to be generally very low. Many single amino acid additions or deletions are likely to be neutral even if the effective population size is as large as that of the budding yeast. This should also apply to substitutions. Selection is much more likely to operate if point mutations affect protein structure by, for example, extending or creating stretches that tend to unfold or interact improperly with membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.