ArticleCellular microbiology2014
Two distinct sensing pathways allow recognition of Klebsiella pneumoniae by Dictyostelium amoebae.
Article in Cellular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Compound K14 inhibits bacterial killing and protease activity in Dictyostelium discoideum phagosomes.PloS one · 2024Article
- Using Functional Annotations to Study Pairwise Interactions in Urinary Tract Infection Communities.Genes · 2021Article
- The Ecology and Evolution of Amoeba-Bacterium Interactions.Applied and environmental microbiology · 2021Review
- Role of LrrkA in the Control of Phagocytosis and Cell Motility inFrontiers in cell and developmental biology · 2021Article
- Dictyostelium discoideum as a non-mammalian biomedical model.Microbial biotechnology · 2021Review
- Article
- Klebsiella pneumoniae infection biology: living to counteract host defences.FEMS microbiology reviews · 2019Review
- Article
- A G-protein-coupled chemoattractant receptor recognizes lipopolysaccharide for bacterial phagocytosis.PLoS biology · 2018Article
- Collective regulation of cell motility using an accurate density-sensing system.Journal of the Royal Society, Interface · 2018Article
- Evaluating Different Virulence Traits ofFrontiers in cellular and infection microbiology · 2018Article
- Vps13F links bacterial recognition and intracellular killing in Dictyostelium.Cellular microbiology · 2017Article
- Chemotactic network responses to live bacteria show independence of phagocytosis from chemoreceptor sensing.eLife · 2017Article
- Eat Prey, Live:Frontiers in immunology · 2017Review
- Intracellular killing of bacteria: is Dictyostelium a model macrophage or an alien?Cellular microbiology · 2014Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recognition of bacteria by metazoans is mediated by receptors that recognize different types of microorganisms and elicit specific cellular responses. The soil amoebae Dictyostelium discoideum feeds upon a variable mixture of environmental bacteria, and it is expected to recognize and adapt to various food sources. To date, however, no bacteria-sensing mechanisms have been described. In this study, we isolated a Dictyostelium mutant (fspA KO) unable to grow in the presence of non-capsulated Klebsiella pneumoniae bacteria, but growing as efficiently as wild-type cells in the presence of other bacteria, such as Bacillus subtilis. fspA KO cells were also unable to respond to K. pneumoniae and more specifically to bacterially secreted folate in a chemokinetic assay, while they responded readily to B. subtilis. Remarkably, both WT and fspA KO cells were able to grow in the presence of capsulated LM21 K. pneumoniae, and responded to purified capsule, indicating that capsule recognition may represent an alternative, FspA-independent mechanism for K. pneumoniae sensing. When LM21 capsule synthesis genes were deleted, growth and chemokinetic response were lost for fspA KO cells, but not for WT cells. Altogether, these results indicate that Dictyostelium amoebae use specific recognition mechanisms to respond to different K. pneumoniae elements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.