ArticleThe Journal of physiology2013
Endogenous and maximal sarcoplasmic reticulum calcium content and calsequestrin expression in type I and type II human skeletal muscle fibres.
Article in The Journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 61 citations in OpenAlex.
- Effects of 8 Weeks of Moderate- or High-Volume Strength Training on Sarcoplasmic Reticulum CaScandinavian journal of medicine & science in sports · 2025Trial
- The influence of sex on the effects of inorganic nitrate supplementation on muscular power and endurance.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Trial
- STIM1 Reduction Prevents Tubular Aggregate Formation and Compromises Muscle Performance in Ageing Mice.Journal of cachexia, sarcopenia and muscle · 2025Article
- Comparison of Phasic Store-Operated Calcium Entry in Rat Slow- and Fast-Twitch Muscle Fibers.Acta physiologica (Oxford, England) · 2025Article
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- A bivalent remipede toxin promotes calcium release via ryanodine receptor activation.Nature communications · 2023Article
- Calcium and strontium contractile activation properties of single skinned skeletal muscle fibres from elderly women 66-90 years of age.Journal of muscle research and cell motility · 2022Article
- CASQ1-related myopathy: The first report from China and the literature review.Clinical case reports · 2022Article
- Measurements of basal d-glucose transport through GLUT1 across the intact plasma membrane of isolated segments from single fast- and slow-twitch skeletal muscle fibres of rat.Acta physiologica (Oxford, England) · 2022Article
- Sarcopenia in pulmonary diseases is associated with elevated sarcoplasmic reticulum stress and myonuclear disorganization.Histochemistry and cell biology · 2022Article
- Structure-function relationships and modifications of cardiac sarcoplasmic reticulum Ca2+-transport.Physiological research · 2021Review
- Ryanodine receptor leak triggers fiber CaScience advances · 2021Article
- Article
- Caffeine as a tool to investigate sarcoplasmic reticulum and intracellular calcium dynamics in human skeletal muscles.Journal of muscle research and cell motility · 2021Review
- Pathological mechanisms of vacuolar aggregate myopathy arising from a Casq1 mutation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Loss of α-actinin-3 during human evolution provides superior cold resilience and muscle heat generation.American journal of human genetics · 2021Article
- Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease.Frontiers in physiology · 2021Review
- Effects of S-glutathionylation on the passive force-length relationship in skeletal muscle fibres of rats and humans.Journal of muscle research and cell motility · 2020Article
- Effects of high-intensity intermittent exercise on the contractile properties of human type I and type II skeletal muscle fibers.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
- A fast, reliable and sample-sparing method to identify fibre types of single muscle fibres.Scientific reports · 2019Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The relationship between sarcoplasmic reticulum (SR) Ca(2+) content and calsequestrin (CSQ) isoforms was investigated in human skeletal muscle. A fibre-lysing assay was used to quantify the endogenous Ca(2+) content and maximal Ca(2+) capacity of the SR in skinned segments of type I and type II fibres from vastus lateralis muscles of young healthy adults. Western blotting of individual fibres showed the great majority contained either all fast or all slow isoforms of myosin heavy chain (MHC), troponins C and I, tropomyosin and SERCA, and that the strontium sensitivity of the force response was closely indicative of the troponin C isoform present. The endogenous SR Ca(2+) content was slightly lower in type I compared to type II fibres (0.76 ± 0.03 and 0.85 ± 0.02 mmol Ca(2+) per litre of fibre, respectively), with virtually all of this Ca(2+) evidently being in the SR, as it could be rapidly released with a caffeine-low [Mg(2+)] solution (only 0.08 ± 0.01 and <0.07 mmol l(-1), respectively, remaining). The maximal Ca(2+) content that could be reached with SR Ca(2+) loading was 1.45 ± 0.04 and 1.79 ± 0.03 mmol l(-1) in type I and type II fibres, respectively (P < 0.05). In non-lysed skinned fibres, where the SR remained functional, repeated cycles of caffeine-induced Ca(2+) release and subsequent Ca(2+) reloading similarly indicated that (i) maximal SR Ca(2+) content was lower in type I fibres than in type II fibres (P < 0.05), and (ii) the endogenous Ca(2+) content represented a greater percentage of maximal content in type I fibres compared to type II fibres (∼59% and 41%, respectively, P < 0.05). Type II fibres were found on average to contain ∼3-fold more CSQ1 and ∼5-fold less CSQ2 than type I fibres (P < 0.001). The findings are consistent with the SR Ca(2+) content characteristics in human type II fibres being primarily determined by the CSQ1 abundance, and in type I fibres by the combined amounts of both CSQ1 and CSQ2.
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