ArticleThe Journal of physiology2013
Optical determination of intracellular water in apoptotic cells.
Article in The Journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Live, spatially-resolved imaging of absolute intracellular water content using holotomography.Biomedical optics express · 2025Article
- Metabolic Energy is Stored in a Homeostatic Trans-Membrane Water Barochemical Gradient.The Journal of membrane biology · 2025Article
- Combined Dielectric-Optical Characterization of Single Cells Using Dielectrophoresis-Imaging Flow Cytometry.Biosensors · 2024Article
- Measurement of protein concentration in bacteria and small organelles under a light transmission microscope.Journal of molecular recognition : JMR · 2024Article
- A Futile Cycle?: Tissue Homeostatic Trans-Membrane Water Co-Transport: Kinetics, Thermodynamics, Metabolic Consequences.bioRxiv : the preprint server for biology · 2024Article
- Rapid and precise tracking of water influx and efflux across cell membranes induced by a pulsed electric field.Biomedical optics express · 2023Article
- Influence of Yokukansan on the refractive index of neuroblastoma cells.Biomedical optics express · 2023Article
- Role of transporters in regulating mammalian intracellular inorganic phosphate.Frontiers in pharmacology · 2023Review
- Comprehensive single-shot biophysical cytometry using simultaneous quantitative phase imaging and Brillouin spectroscopy.Scientific reports · 2022Article
- Article
- A Reverse-Osmosis Model of Apoptotic Shrinkage.Frontiers in cell and developmental biology · 2020Article
- Death for life: a path from apoptotic signaling to tissue-scale effects of apoptotic epithelial extrusion.Cellular and molecular life sciences : CMLS · 2019Review
- Presumptive TRP channel CED-11 promotes cell volume decrease and facilitates degradation of apoptotic cells inProceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Intracellular water plays a critical role in apoptotic and necrotic cell death. We describe a method for quantifying cell water by application of two previously described variants of transmission microscopy. By taking two axially displaced brightfield images, the phase shift of the transmitted wave was computed using the transport-of-intensity equation. At the same time, cell thickness was determined by transmission through an externally applied dye ('transmission-through-dye' microscopy); switching between these two imaging modalities was accomplished by simply changing the illumination wavelength. The sets of data thus obtained allow computation of the refractive index and cell water content within individual cells. The method was illustrated using cells treated with apoptotic agents staurosporine and actinomycin D and with necrosis inducer ionomycin. Water imaging allows discrimination between apoptotic volume decrease due to dehydration from that due to detachment of apoptotic bodies and can be used on samples where cell volume determination alone would be difficult or insufficient.
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Registered trials
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