ArticleJournal of experimental botany2013
Biochemical and physiological characterization of fut4 and fut6 mutants defective in arabinogalactan-protein fucosylation in Arabidopsis.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 74 citations in OpenAlex.
- The Transcription Factor PsLBD42 Enhances Drought Tolerance Through Regulating the Fucosyltransferase PsFUT2 to Sustain Root Growth in Poplar.Plant, cell & environment · 2026Article
- Cell wall fucosylation in Arabidopsis influences control of leaf water loss and alters stomatal development and mechanical properties.Journal of experimental botany · 2023Article
- Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum.Microbial cell factories · 2023Article
- Functional characterization of hydroxyproline-O-galactosyltransferases for Arabidopsis arabinogalactan-protein synthesis.BMC plant biology · 2021Article
- A Pipeline towards the Biochemical Characterization of theInternational journal of molecular sciences · 2021Article
- CRISPR-Cas9 multiplex genome editing of the hydroxyproline-O-galactosyltransferase gene family alters arabinogalactan-protein glycosylation and function in Arabidopsis.BMC plant biology · 2021Article
- Biochemical and Functional Characterization of GALT8, anFrontiers in plant science · 2021Article
- Cracking the "Sugar Code": A Snapshot ofFrontiers in plant science · 2021Review
- Article
- Calcium Binding by Arabinogalactan Polysaccharides Is Important for Normal Plant Development.The Plant cell · 2020Article
- A Glycan Array-Based Assay for the Identification and Characterization of Plant Glycosyltransferases.Angewandte Chemie (International ed. in English) · 2020Article
- Three Decades of Advances in Arabinogalactan-Protein Biosynthesis.Frontiers in plant science · 2020Review
- CRISPR/Cas9 Genome Editing Technology: A Valuable Tool for Understanding Plant Cell Wall Biosynthesis and Function.Frontiers in plant science · 2020Review
- On the Potential Function of Type II ArabinogalactanFrontiers in plant science · 2020Review
- An important role of l-fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity.The New phytologist · 2019Article
- A Hybrid Approach Enabling Large-Scale Glycomic Analysis of Post-Golgi Vesicles Reveals a Transport Route for Polysaccharides.The Plant cell · 2019Article
- Gene Regulatory Networks for the Haploid-to-Diploid Transition ofPlant physiology · 2017Article
- The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development.Nature communications · 2016Article
- Extensin and Arabinogalactan-Protein Biosynthesis: Glycosyltransferases, Research Challenges, and Biosensors.Frontiers in plant science · 2016Review
- Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabinogalactan-proteins (AGPs) are highly glycosylated hydroxyproline-rich glycoproteins present in plant cell walls. AGPs are characterized by arabinose-/galactose-rich side chains, which define their interactive molecular surface. Fucose residues are found in some dicotyledon AGPs, and AGP fucosylation is developmentally regulated. We previously identified Arabidopsis thaliana FUT4 and FUT6 genes as AGP-specific fucosyltransferases (FUTs) based on their enzymatic activities when heterologously expressed in tobacco (Nicotiana tabacum) BY2 suspension-cultured cells. Here, the functions of FUT4 and FUT6 and the physiological roles of fucosylated AGPs were further investigated using Arabidopsis fut4, fut6, and fut4/fut6 mutant plants. All mutant plants showed no phenotypic differences compared to wild-type plants under physiological conditions, but showed reduced root growth in the presence of elevated NaCl. However, roots of wild-type and fut4 mutant plants contained terminal fucose epitopes, which were absent in fut6 and fut4/fut6 mutant plants as indicated by eel lectin staining. Monosaccharide analysis showed fucose was present in wild-type leaf and root AGPs, but absent in fut4 leaf AGPs and in fut4/fut6 double mutant leaf and root AGPs, indicating that FUT4 was required for fucosylation of leaf AGPs while both FUT4 and FUT6 contributed to fucosylation of root AGPs. Glycome profiling of cell wall fractions from mutant roots and leaves showed distinct glycome profiles compared to wild-type plants, indicating that fucosyl residues on AGPs may regulate intermolecular interactions between AGPs and other wall components. The current work exemplifies the possibilities of refinement of cell wall structures by manipulation of a single or a few cell wall biosynthetic genes.
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