ArticleJournal of experimental botany2013
Molecular and biochemical analysis of the first ARA6 homologue, a RAB5 GTPase, from green algae.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Recovery of the cortical chloroplast layer in the green algaFrontiers in plant science · 2025Article
- Review
- Uptake of oomycete RXLR effectors into host cells by clathrin-mediated endocytosis.The Plant cell · 2023Article
- RAB GTPases and SNAREs at the trans-Golgi network in plants.Journal of plant research · 2022Review
- Heterologous Expression of ArabidopsisFrontiers in genetics · 2022Article
- Fluid-phase and membrane markers reveal spatio-temporal dynamics of membrane traffic and repair in the green alga Chara australis.Protoplasma · 2021Article
- Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase.Scientific reports · 2018Article
- Article
- Arabidopsis RabF1 (ARA6) Is Involved in Salt Stress and Dark-Induced Senescence (DIS).International journal of molecular sciences · 2017Article
- Clathrin inFrontiers in plant science · 2017Article
- PIN2-like proteins may contribute to the regulation of morphogenetic processes during spermatogenesis in Chara vulgaris.Plant cell reports · 2016Article
- Plasmodium Rab5b is secreted to the cytoplasmic face of the tubovesicular network in infected red blood cells together with N-acylated adenylate kinase 2.Malaria journal · 2016Article
- Is Wortmannin-Induced Reorganization of the trans-Golgi Network the Key to Explain Charasome Formation?Frontiers in plant science · 2016Article
- Functional analyses of the plant-specific C-terminal region of VPS9a: the activating factor for RAB5 in Arabidopsis thaliana.Journal of plant research · 2016Article
- Convoluted Plasma Membrane Domains in the Green Alga Chara are Depleted of Microtubules and Actin Filaments.Plant & cell physiology · 2015Article
- Molecular Analysis and Localization of CaARA7 a Conventional RAB5 GTPase from Characean Algae.Traffic (Copenhagen, Denmark) · 2015Article
- An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology.Frontiers in plant science · 2014Article
- Vesicular trafficking in characean green algae and the possible involvement of a VAMP72-family protein.Plant signaling & behavior · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
RAB5 GTPases are important regulators of endosomal membrane traffic in yeast, plants, and animals. A specific subgroup of this family, the ARA6 group, has been described in land plants including bryophytes, lycophytes, and flowering plants. Here, we report on the isolation of an ARA6 homologue in a green alga. CaARA6 (CaRABF1) from Chara australis, a member of the Characeae that is a close relative of land plants, encodes a polypeptide of 237 aa with a calculated molecular mass of 25.4 kDa, which is highly similar to ARA6 members from Arabidopsis thaliana and other land plants and has GTPase activity. When expressed in Nicotiana benthamiana leaf epidermal cells, fluorescently tagged CaARA6 labelled organelles with diameters between 0.2 and 1.2 µm, which co-localized with fluorescently tagged AtARA6 known to be present on multivesicular endosomes. Mutations in the membrane-anchoring and GTP-binding sites altered the localization of CaARA6 comparable to that of A. thaliana ARA6 (RABF1). In characean internodal cells, confocal immunofluorescence and immunogold electron microscopy with antibodies against AtARA6 and CaARA6 revealed ARA6 epitopes not only at multivesicular endosomes but also at the plasma membrane, including convoluted domains (charasomes), and at the trans-Golgi network. Our findings demonstrate that ARA6-like proteins have a more ancient origin than previously thought. They indicate further that ARA6-like proteins could have different functions in spite of the high similarity between characean algae and flowering plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.