ArticleInfection and immunity2014
Insulin treatment modulates the host immune system to enhance Pseudomonas aeruginosa wound biofilms.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 69 citations in OpenAlex.
- Rhein-induced free fatty acid generation cooperates with silver ions for biofilm-infected diabetic wound healing.Signal transduction and targeted therapy · 2026Article
- Insulin Affects Biological Behaviours ofPharmaceuticals (Basel, Switzerland) · 2026Article
- Natural and synthetic chemicals acting as enhancers of bacterial pathogenic success within the human host.Frontiers in cellular and infection microbiology · 2026Review
- Proactive Strategies to Prevent Biofilm-Associated Infections: From Mechanistic Insights to Clinical Translation.Microorganisms · 2025Review
- Global genome analysis identifies glycolipids and lipoteichoic acid alanylation as contributors to group B streptococcal diabetic wound infection.Cell reports · 2025Article
- Interplay Between Insulin Resistance and Immune Dysregulation in Type 2 Diabetes Mellitus: Implications for Therapeutic Interventions.ImmunoTargets and therapy · 2025Review
- What Is a Biofilm? Lessons Learned from Interactions with Immune Cells.International journal of molecular sciences · 2024Review
- Virulence characteristics of Gram-positive bacteria isolated from diabetic foot ulcers.FEMS microbes · 2024Article
- Protein-modified nanomaterials: emerging trends in skin wound healing.Discover nano · 2023Review
- Pathogenesis of thePathogens (Basel, Switzerland) · 2022Review
- Immunometabolism in biofilm infection: lessons from cancer.Molecular medicine (Cambridge, Mass.) · 2022Review
- Challenges and innovations in treating chronic and acute wound infections: from basic science to clinical practice.Burns & trauma · 2022Review
- Article
- Detection of bacterial fluorescence from in vivo wound biofilms using a point-of-care fluorescence imaging device.International wound journal · 2021Article
- Utilizing glycoside hydrolases to improve the quantitation and visualization of biofilm bacteria.Biofilm · 2020Article
- Review
- The evolution of virulence inProceedings. Biological sciences · 2020Article
- Bacteriophage therapy against Pseudomonas aeruginosa biofilms: a review.Annals of clinical microbiology and antimicrobials · 2020Review
- It takes two for chronic wounds to heal: dispersing bacterial biofilm and modulating inflammation with dual action plasma coatings.RSC advances · 2020Article
- Differential Efficacy of Glycoside Hydrolases to Disperse Biofilms.Frontiers in cellular and infection microbiology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diabetes affects 25.8 million people in the United States, or 8.3% of the population, and these numbers are even higher in developing countries. Diabetic patients are more susceptible to the development of chronic wounds with debilitating bacterial infections than nondiabetics. Previously, we compared the ability of the opportunistic pathogen Pseudomonas aeruginosa to cause biofilm-associated infections in chronic wounds of diabetic and nondiabetic mice (C. Watters, K. DeLeon, U. Trivedi, J. A. Griswold, M. Lyte, K. J. Hampel, M. J. Wargo, and K. P. Rumbaugh, Med. Microbiol. Immunol. 202:131-141, 2013). Unexpectedly, we observed that insulin-treated diabetic mice had significantly more biofilm in their wounds, which correlated with higher antibiotic tolerance. Here, we investigated whether insulin treatment modulates the diabetic immune system to favor P. aeruginosa biofilm formation. Utilizing a murine chronic wound model, we found that DNA protected P. aeruginosa in the wounds of insulin-treated diabetic mice from antibiotic treatment. We also observed increased numbers of neutrophils, reduced numbers of macrophages, and increased cell death in the wounds of diabetic mice on insulin therapy. Taken together, these data suggest that high levels of lysed neutrophils in the wounds of diabetic mice on insulin, combined with fewer macrophages to remove the cellular debris, contribute to increased DNA levels, which enhance P. aeruginosa biofilms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.