ArticleMolecules (Basel, Switzerland)2013
Comparative analysis of click chemistry mediated activity-based protein profiling in cell lysates.
Article in Molecules (Basel, Switzerland), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Development of a novel assay for antigen presentation measurement.Scientific reports · 2025Article
- Click Chemistry for Target Engagement Studies.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Click Chemistry in Detecting Protein Modification.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Improved deconvolution of natural products' protein targets using diagnostic ions from chemical proteomics linkers.Beilstein journal of organic chemistry · 2024Review
- Cell-selective proteomics segregates pancreatic cancer subtypes by extracellular proteins in tumors and circulation.Nature communications · 2023Article
- Genetically encoded chemical crosslinking of carbohydrate.Nature chemistry · 2023Article
- Methyltetrazine as a small live-cell compatible bioorthogonal handle for imaging enzyme activitiesRSC chemical biology · 2022Article
- Application of a Fluorescence Anisotropy-Based Assay to Quantify Transglutaminase 2 Activity in Cell Lysates.International journal of molecular sciences · 2022Article
- Review
- Bioorthogonal Reactions in Activity-Based Protein Profiling.Molecules (Basel, Switzerland) · 2020Review
- Development of activity-based probes for the protein deacylase Sirt1.Bioorganic chemistry · 2020Article
- Mass Spectrometric Method for the Unambiguous Profiling of Cellular Dynamic Glycosylation.ACS chemical biology · 2020Article
- Monitoring global changes in chromatin compaction states upon localized DNA damage with tools of fluorescence anisotropy.Molecular biology of the cell · 2020Article
- Unbiased Identification of the Liposome Protein Corona using Photoaffinity-based Chemoproteomics.ACS central science · 2020Article
- Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.Journal of proteome research · 2018Article
- A strategy for dual inhibition of the proteasome and fatty acid synthase with belactosin C-orlistat hybrids.Bioorganic & medicinal chemistry · 2017Article
- Comparative analysis of Cu (I)-catalyzed alkyne-azide cycloaddition (CuAAC) and strain-promoted alkyne-azide cycloaddition (SPAAC) in O-GlcNAc proteomics.Electrophoresis · 2016Article
- Shedding light on protein folding, structural and functional dynamics by single molecule studies.Molecules (Basel, Switzerland) · 2014Review
- Bioorthogonal click chemistry to assay mu-opioid receptor palmitoylation using 15-hexadecynoic acid and immunoprecipitation.Analytical biochemistry · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activity-based protein profiling uses chemical probes that covalently attach to active enzyme targets. Probes with conventional tags have disadvantages, such as limited cell permeability or steric hindrance around the reactive group. A tandem labeling strategy with click chemistry is now widely used to study enzyme targets in situ and in vivo. Herein, the probes are reacted in live cells, whereas the ensuing detection by click chemistry takes place in cell lysates. We here make a comparison of the efficiency of the activity-based tandem labeling strategy by using Cu(I)-catalyzed and strain-promoted click chemistry, different ligands and different lysis conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.