ArticleJournal of molecular biology2014
Structure-function analysis of the DNA translocating portal of the bacteriophage T4 packaging machine.
Article in Journal of molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Cryo-EM structure of bacteriophage Nocturne116: insights into the architecture of c2-likeJournal of virology · 2026Article
- In situ structures of the portal-neck-tail complex of bacteriophage T4 inform a viral genome positioning mechanism.Nature communications · 2026Article
- Cryo-EM structures of bacteriophage T4 portal-neck assembly intermediates reveal a viral genome retention mechanism.Nature communications · 2026Article
- Mutagenesis and functional analysis of the varicella-zoster virus portal protein.Journal of virology · 2024Article
- Cryo-EM Structures of Two Bacteriophage Portal Proteins Provide Insights for Antimicrobial Phage Engineering.Viruses · 2021Article
- The remarkable viral portal vertex: structure and a plausible model for mechanism.Current opinion in virology · 2021Review
- Cryo-EM structure in situ reveals a molecular switch that safeguards virus against genome loss.eLife · 2020Article
- Portal Protein: The Orchestrator of Capsid Assembly for the dsDNA Tailed Bacteriophages and Herpesviruses.Annual review of virology · 2019Review
- Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism.Nature communications · 2019Article
- May the Road Rise to Meet You: DNA Deformation May Drive DNA Translocation.Biophysical journal · 2019Article
- Nucleotide-dependent DNA gripping and an end-clamp mechanism regulate the bacteriophage T4 viral packaging motor.Nature communications · 2018Article
- Protein structure model refinement in CASP12 using short and long molecular dynamics simulations in implicit solvent.Proteins · 2018Article
- Prediction of Local Quality of Protein Structure Models Considering Spatial Neighbors in Graphical Models.Scientific reports · 2017Article
- DNA Scrunching in the Packaging of Viral Genomes.The journal of physical chemistry. B · 2016Article
- Exclusion of small terminase mediated DNA threading models for genome packaging in bacteriophage T4.Nucleic acids research · 2016Article
- Mechanisms of DNA Packaging by Large Double-Stranded DNA Viruses.Annual review of virology · 2015Review
- Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution.Nature communications · 2015Article
- Structural rearrangements in the phage head-to-tail interface during assembly and infection.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The varicella-zoster virus portal protein is essential for cleavage and packaging of viral DNA.Journal of virology · 2014Article
- Temperate Streptococcus thermophilus phages expressing superinfection exclusion proteins of the Ltp type.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Tailed bacteriophages and herpesviruses consist of a structurally well conserved dodecameric portal at a special 5-fold vertex of the capsid. The portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. Although the structures of portals from phages φ29, SPP1, and P22 have been determined, their mechanistic roles have not been well understood. Structural analysis of phage T4 portal (gp20) has been hampered because of its unusual interaction with the Escherichia coli inner membrane. Here, we predict atomic models for the T4 portal monomer and dodecamer, and we fit the dodecamer into the cryo-electron microscopy density of the phage portal vertex. The core structure, like that from other phages, is cone shaped with the wider end containing the "wing" and "crown" domains inside the phage head. A long "stem" encloses a central channel, and a narrow "stalk" protrudes outside the capsid. A biochemical approach was developed to analyze portal function by incorporating plasmid-expressed portal protein into phage heads and determining the effect of mutations on head assembly, DNA translocation, and virion production. We found that the protruding loops of the stalk domain are involved in assembling the DNA packaging motor. A loop that connects the stalk to the channel might be required for communication between the motor and the portal. The "tunnel" loops that project into the channel are essential for sealing the packaged head. These studies established that the portal is required throughout the DNA packaging process, with different domains participating at different stages of genome packaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.