ArticleBiomaterials2014
TGF-β3-induced chondrogenesis in co-cultures of chondrocytes and mesenchymal stem cells on biodegradable scaffolds.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 102 citations in OpenAlex.
- Extracellular matrix assembly during chondrogenic differentiation of amniotic fluid- and adipose-derived mesenchymal stromal cells under TGF-β3 induction.Journal of molecular histology · 2026Article
- The Effect of Physical, Biochemical, and Electrical Culture Conditions on Articular Chondrocytes Used for Cartilage Tissue Engineering: A Narrative Review.International journal of molecular sciences · 2026Review
- Avenanthramide C and chondroitin sulphate promote chondrogenic differentiation of adipose-derived mesenchymal stem cells.Scientific reports · 2026Article
- PET/EVOH nonwoven fabrics supportRegenerative therapy · 2026Article
- Signaling network exploration of microRNA140-5p in response to TMJ-OA pathological changes.Scientific reports · 2026Article
- Combined TGF-β3 and FGF-2 Stimulation Enhances Chondrogenic Potential of Ovine Bone Marrow-Derived MSCs.Cells · 2025Article
- The phenotypic characterization of mouse floxedRegenerative therapy · 2025Article
- Most vexing problem in pediatric fractures: Epiphyseal fractures.World journal of orthopedics · 2025Review
- Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel.Biotechnology letters · 2024Article
- Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment.Cell communication and signaling : CCS · 2024Review
- 3D Bioprinting in Otolaryngology: A Review.Advanced healthcare materials · 2023Review
- Advanced 3D dynamic culture system with transforming growth factor-β3 enhances production of potent extracellular vesicles with modified protein cargoes via upregulation of TGF-β signaling.Journal of advanced research · 2023Article
- Nestin+ Peyer's patch resident MSCs enhance healing of inflammatory bowel disease through IL-22-mediated intestinal epithelial repair.Cell proliferation · 2023Article
- Human gelatin-based composite hydrogels for osteochondral tissue engineering and their adaptation into bioinks for extrusion, inkjet, and digital light processing bioprinting.Biofabrication · 2022Article
- Bioprinting of Chondrocyte Stem Cell Co-Cultures for Auricular Cartilage Regeneration.ACS omega · 2022Article
- Review
- Gelatin methacryloyl as environment for chondrocytes and cell delivery to superficial cartilage defects.Journal of tissue engineering and regenerative medicine · 2022Article
- Naringin in the repair of knee cartilage injury via the TGF-β/ALK5/Smad2/3 signal transduction pathway combined with an acellular dermal matrix.Journal of orthopaedic translation · 2022Article
- Comparing the immunomodulatory properties of equine BM-MSCs culture expanded in autologous platelet lysate, pooled platelet lysate, equine serum and fetal bovine serum supplemented culture media.Frontiers in veterinary science · 2022Article
- The Chondrogenic Potential of First-Trimester and Term Placental Mesenchymal Stem/Stromal Cells.Cartilage · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In this work, it was hypothesized that co-cultures of articular chondrocytes (ACs) and mesenchymal stem cells (MSCs) would exhibit enhanced sensitivity to chondrogenic stimuli, such as TGF-β3, and would require a reduced concentration of TGF-β3 to achieve an equivalent level of chondrogenesis compared to monocultures of each cell type. Furthermore, it was hypothesized that compared to monocultures, the chondrogenic phenotype of AC/MSC co-cultures would be more stable upon the removal of TGF-β3 from the culture medium. These hypotheses were investigated by culturing ACs and MSCs alone and in a 1:3 ratio on electrospun poly(ε-caprolactone) scaffolds. All cell populations were cultured for two weeks with 0, 1, 3, or 10 ng/ml of TGF-β3. After two weeks growth factor supplementation was removed, and the constructs were cultured for two additional weeks. Cell proliferation, extracellular matrix production, and chondrogenic gene expression were evaluated after two and four weeks. The results demonstrated that co-cultures of ACs and MSCs require a reduced concentration and duration of TGF-β3 exposure to achieve an equivalent level of chondrogenesis compared to AC or MSC monocultures. Thus, the present work implicates that the promise of co-cultures for cartilage engineering is enhanced by their robust phenotype and heightened sensitivity to TGF-β3.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.