ArticlePloS one2013
Improving acetate tolerance of Escherichia coli by rewiring its global regulator cAMP receptor protein (CRP).
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Demonstration and technoeconomic analysis of dodecanol production from acetate using metabolically engineered Escherichia coli.Metabolic engineering · 2026Article
- Alternate routes to acetate tolerance lead to varied isoprenol production from mixed carbon sources inApplied and environmental microbiology · 2025Article
- Identification and monitoring of cell heterogeneity from plasmid recombination during limonene production.Applied microbiology and biotechnology · 2025Article
- CRP and IHF act as host regulators in Royal Jelly's antibacterial activity.Scientific reports · 2024Article
- Medium Chain Length Polyhydroxyalkanoate Production by Engineered Pseudomonas gessardii Using Acetate-formate as Carbon Sources.Journal of microbiology (Seoul, Korea) · 2024Article
- Application of Acetate as a Substrate for the Production of Value-Added Chemicals inMicroorganisms · 2024Review
- Minimizing acetate formation from overflow metabolism inFrontiers in bioengineering and biotechnology · 2024Article
- Advance of tolerance engineering on microbes for industrial production.Synthetic and systems biotechnology · 2023Review
- Synthesis of isobutanol using acetate as sole carbon source in Escherichia coli.Microbial cell factories · 2023Article
- Synthetic Biology Toolkit for a New Species ofMicroorganisms · 2023Article
- Citrate Synthase Overexpression ofACS omega · 2023Article
- Scale-Up of Capsular Polysaccharide Production Process byBioengineering (Basel, Switzerland) · 2022Article
- Enhanced Acetate Tolerance and Recombinant Protein Accumulation inIranian journal of biotechnology · 2022Article
- The Potential of Sequential Fermentations in Converting C1 Substrates to Higher-Value Products.Frontiers in microbiology · 2022Review
- Microbial Upgrading of Acetate into Value-Added Products-Examining Microbial Diversity, Bioenergetic Constraints and Metabolic Engineering Approaches.International journal of molecular sciences · 2020Review
- Multi-Path Optimization for Efficient Production of 2'-Fucosyllactose in an EngineeredFrontiers in bioengineering and biotechnology · 2020Article
- Acetate Metabolism and the Inhibition of Bacterial Growth by Acetate.Journal of bacteriology · 2019Article
- Production of medium chain length polyhydroxyalkanoate from acetate by engineered Pseudomonas putida KT2440.Journal of industrial microbiology & biotechnology · 2019Article
- Engineering Escherichia coli to grow constitutively on D-xylose using the carbon-efficient Weimberg pathway.Microbiology (Reading, England) · 2018Article
- Improving E. coli growth performance by manipulating small RNA expression.Microbial cell factories · 2017Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of acetate exceeding 5 g/L is a major concern during E. coli fermentation due to its inhibitory effect on cell growth, thereby limiting high-density cell culture and recombinant protein production. Hence, engineered E. coli strains with enhanced acetate tolerance would be valuable for these bioprocesses. In this work, the acetate tolerance of E. coli was much improved by rewiring its global regulator cAMP receptor protein (CRP), which is reported to regulate 444 genes. Error-prone PCR method was employed to modify crp and the mutagenesis libraries (~3×10(6)) were subjected to M9 minimal medium supplemented with 5-10 g/L sodium acetate for selection. Mutant A2 (D138Y) was isolated and its growth rate in 15 g/L sodium acetate was found to be 0.083 h(-1), much higher than that of the control (0.016 h(-1)). Real-time PCR analysis via OpenArray(®) system revealed that over 400 CRP-regulated genes were differentially expressed in A2 with or without acetate stress, including those involved in the TCA cycle, phosphotransferase system, etc. Eight genes were chosen for overexpression and the overexpression of uxaB was found to lead to E. coli acetate sensitivity.
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