ArticlePloS one2013
Engineering salidroside biosynthetic pathway in hairy root cultures of Rhodiola crenulata based on metabolic characterization of tyrosine decarboxylase.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 67 citations in OpenAlex.
- Early Developmental Regulation of Tyrosine Decarboxylase in Olive Fruits and Its Impact on Virgin Olive Oil Phenolics.Journal of agricultural and food chemistry · 2026Article
- Quality differentiation and salidroside biosynthesis inFrontiers in plant science · 2026Article
- Complete pathway elucidation of echinacoside in Cistanche tubulosa and de novo biosynthesis of phenylethanoid glycosides.Nature communications · 2025Article
- The Untapped Potential of Hairy Root Cultures and Their Multiple Applications.International journal of molecular sciences · 2024Review
- Potential of natural drug modulation of endoplasmic reticulum stress in the treatment of myocardial injury.Journal of pharmaceutical analysis · 2024Review
- Functional and Physiological Characterization of Tyrosine Decarboxylases fromInternational journal of molecular sciences · 2024Article
- Functional differentiation of olive PLP_deC genes: insights into metabolite biosynthesis and genetic improvement at the whole-genome level.Plant cell reports · 2024Article
- Hairy Root Cultures as a Source of Phenolic Antioxidants: Simple Phenolics, Phenolic Acids, Phenylethanoids, and Hydroxycinnamates.International journal of molecular sciences · 2023Review
- Hydroxytyrosol Interference with Inflammaging via Modulation of Inflammation and Autophagy.Nutrients · 2023Review
- Redesigning plant specialized metabolism with supervised machine learning using publicly available reactome data.Computational and structural biotechnology journal · 2023Review
- Article
- Genome-wide Identification and Expression Analysis ofFrontiers in genetics · 2022Article
- Identification and Functional Characterization of Genes Encoding Phenylacetaldehyde Reductases That Catalyze the Last Step in the Biosynthesis of Hydroxytyrosol in Olive.Plants (Basel, Switzerland) · 2021Article
- Article
- Using Gene Expression to Study Specialized Metabolism-A Practical Guide.Frontiers in plant science · 2020Review
- An Aromatic Aldehyde Synthase Controls the Synthesis of Hydroxytyrosol Derivatives Present in Virgin Olive Oil.Antioxidants (Basel, Switzerland) · 2019Article
- Identification and Microbial Production of the Raspberry Phenol Salidroside that Is Active against Huntington's Disease.Plant physiology · 2019Article
- Identification of Glutathione Peroxidase (GPX) Gene Family inInternational journal of molecular sciences · 2018Article
- Salidroside mitigates hypoxia/reoxygenation injury by alleviating endoplasmic reticulum stress‑induced apoptosis in H9c2 cardiomyocytes.Molecular medicine reports · 2018Article
- A Novel UDP-Glycosyltransferase ofBioMed research international · 2018Article
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tyrosine decarboxylase initializes salidroside biosynthesis. Metabolic characterization of tyrosine decarboxylase gene from Rhodiola crenulata (RcTYDC) revealed that it played an important role in salidroside biosynthesis. Recombinant 53 kDa RcTYDC converted tyrosine into tyramine. RcTYDC gene expression was induced coordinately with the expression of RcUDPGT (the last gene involved in salidroside biosynthesis) in SA/MeJA treatment; the expression of RcTYDC and RcUDPGT was dramatically upregulated by SA, respectively 49 folds and 36 folds compared with control. MeJA also significantly increased the expression of RcTYDC and RcUDPGT in hairy root cultures. The tissue profile of RcTYDC and RcUDPGT was highly similar: highest expression levels found in stems, higher expression levels in leaves than in flowers and roots. The gene expressing levels were consistent with the salidroside accumulation levels. This strongly suggested that RcTYDC played an important role in salidroside biosynthesis in R. crenulata. Finally, RcTYDC was used to engineering salidroside biosynthetic pathway in R. crenulata hairy roots via metabolic engineering strategy of overexpression. All the transgenic lines showed much higher expression levels of RcTYDC than non-transgenic one. The transgenic lines produced tyramine, tyrosol and salidroside at higher levels, which were respectively 3.21-6.84, 1.50-2.19 and 1.27-3.47 folds compared with the corresponding compound in non-transgenic lines. In conclusion, RcTYDC overexpression promoted tyramine biosynthesis that facilitated more metabolic flux flowing toward the downstream pathway and as a result, the intermediate tyrosol was accumulated more that led to the increased production of the end-product salidroside.
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