ArticleAndrology2013
Hormone suppression with GnRH antagonist promotes spermatogenic recovery from transplanted spermatogonial stem cells in irradiated cynomolgus monkeys.
Article in Andrology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Single-Nucleus RNA-Seq Reveals Spermatogonial Stem Cell Developmental Pattern in Shaziling Pigs.Biomolecules · 2024Article
- Molecular phenotyping of domestic cat (Theriogenology wild · 2023Article
- Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Models and Methods for Evaluating Regeneration of Spermatogenesis After Cytotoxic Treatments.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Male fertility preservation and restoration strategies for patients undergoing gonadotoxic therapies†.Biology of reproduction · 2022Review
- Progress in germline stem cell transplantation in mammals and the potential usage.Reproductive biology and endocrinology : RB&E · 2022Review
- Pretreatment with gonadotropin-releasing hormone antagonist protects against chemotherapy-induced testicular damage 'in mice.Therapeutic advances in medical oncology · 2022Article
- Article
- Restoration of functional sperm production in irradiated pubertal rhesus monkeys by spermatogonial stem cell transplantation.Andrology · 2020Article
- Approaches and Technologies in Male Fertility Preservation.International journal of molecular sciences · 2020Review
- Oncofertility: Pharmacological Protection and Immature Testicular Tissue (ITT)-Based Strategies for Prepubertal and Adolescent Male Cancer Patients.International journal of molecular sciences · 2019Review
- Transcriptional Profiling of Non-Human Primate Lymphoid Organ Responses to Total-Body Irradiation.Radiation research · 2019Article
- Effect of hormone modulations on donor-derived spermatogenesis or colonization after syngeneic and xenotransplantation in mice.Andrology · 2019Article
- Establishment of an electroporation-mediated gene delivery system in porcine spermatogonial stem cells.In vitro cellular & developmental biology. Animal · 2019Article
- Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche.Nature communications · 2018Article
- Donor spermatogenesis in de novo formed seminiferous tubules from transplanted testicular cells in rhesus monkey testis.Human reproduction (Oxford, England) · 2018Article
- Cancer treatment in childhood and testicular function: the importance of the somatic environment.Endocrine connections · 2018Review
- Process and Pitfalls of Sperm Cryopreservation.Journal of clinical medicine · 2017Review
- Molecular and cellular profiling of acute responses to total body radiation exposure in ovariectomized female cynomolgus macaques.International journal of radiation biology · 2015Article
- Granulocyte colony-stimulating factor prevents loss of spermatogenesis after sterilizing busulfan chemotherapy.Fertility and sterility · 2015Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Hormone suppression given before or after cytotoxic treatment stimulates the recovery of spermatogenesis from endogenous and transplanted spermatogonial stem cells (SSC) and restores fertility in rodents. To test whether the combination of hormone suppression and transplantation could enhance the recovery of spermatogenesis in primates, we irradiated (7 Gy) the testes of 12 adult cynomolgus monkeys and treated six of them with gonadotropin-releasing hormone antagonist (GnRH-ant) for 8 weeks. At the end of this treatment, we transfected cryopreserved testicular cells with green fluorescent protein-lentivirus and autologously transplanted them back into one of the testes. The only significant effect of GnRH-ant treatment on endogenous spermatogenesis was an increase in the percentage of tubules containing differentiated germ cells (tubule differentiation index; TDI) in the sham-transplanted testes of GnRH-ant-treated monkeys compared with radiation-only monkeys at 24 weeks after irradiation. Although transplantation alone after irradiation did not significantly increase the TDI, detection of lentiviral DNA in the spermatozoa of one radiation-only monkey indicated that some transplanted cells colonized the testis. However, the combination of transplantation and GnRH-ant clearly stimulated spermatogenic recovery as evidenced by several observations in the GnRH-ant-treated monkeys receiving transplantation: (i) significant increases (~20%) in the volume and weight of the testes compared with the contralateral sham-transplanted testes and/or to the transplanted testes of the radiation-only monkeys; (ii) increases in TDI compared to the transplanted testes of radiation-only monkeys at 24 weeks (9.6% vs. 2.9%; p = 0.05) and 44 weeks (16.5% vs. 6.1%, p = 0.055); (iii) detection of lentiviral sequences in the spermatozoa or testes of five of the GnRH-ant-treated monkeys and (iv) significantly higher sperm counts than in the radiation-only monkeys. Thus hormone suppression enhances spermatogenic recovery from transplanted SSC in primates and may be a useful tool in conjunction with spermatogonial transplantation to restore fertility in men after cancer treatment.
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