ArticleApplied and environmental microbiology2013
The novel bacterial N-demethylase PdmAB is responsible for the initial step of N,N-dimethyl-substituted phenylurea herbicide degradation.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 51 citations in OpenAlex.
- Molecular bioindicators for assessing environmental impacts of emerging contaminants.Fundamental research · 2026Review
- Molecular Monitoring in Soil Bioremediation: From Genetic Potential to Verified Pathway Operation.International journal of molecular sciences · 2026Review
- Exploring Large Protein Sequence Space through Homology- and Representation-based Hierarchical Clustering.Molecular biology and evolution · 2025Article
- Identification, characterization, and distribution of novel amidase geneApplied and environmental microbiology · 2024Article
- Phytoremediation of isoproturon-contaminated sites by transgenic soybean.Plant biotechnology journal · 2023Article
- Potential and limitations for monitoring of pesticide biodegradation at trace concentrations in water and soil.World journal of microbiology & biotechnology · 2022Review
- The Novel Amidase PcnH Initiates the Degradation of Phenazine-1-Carboxamide in Sphingomonas histidinilytica DS-9.Applied and environmental microbiology · 2022Article
- Carbamate C-N Hydrolase GeneApplied and environmental microbiology · 2020Article
- Insights into the Function and Horizontal Transfer of Isoproturon Degradation Genes (Applied and environmental microbiology · 2020Article
- TonB-Dependent Transporters in Sphingomonads: Unraveling Their Distribution and Function in Environmental Adaptation.Microorganisms · 2020Review
- Isolation and characterization of a novelF1000Research · 2020Article
- Genome Organization and Adaptive Potential of Archetypal Organophosphate Degrading Sphingobium fuliginis ATCC 27551.Genome biology and evolution · 2019Article
- The human gut chemical landscape predicts microbe-mediated biotransformation of foods and drugs.eLife · 2019Article
- An Amidase Gene,Applied and environmental microbiology · 2018Article
- Enhanced and Complete Removal of Phenylurea Herbicides by Combinational Transgenic Plant-Microbe Remediation.Applied and environmental microbiology · 2018Article
- Degradation of Diphenyl Ether in Sphingobium phenoxybenzoativorans SC_3 Is Initiated by a Novel Ring Cleavage Dioxygenase.Applied and environmental microbiology · 2017Article
- Isolation and characterization of Bradyrhizobium sp. SR1 degrading two β-triketone herbicides.Environmental science and pollution research international · 2016Article
- Metabolic pathway involved in 2-methyl-6-ethylaniline degradation by Sphingobium sp. strain MEA3-1 and cloning of the novel flavin-dependent monooxygenase system meaBA.Applied and environmental microbiology · 2015Article
- PbaR, an IclR family transcriptional activator for the regulation of the 3-phenoxybenzoate 1',2'-dioxygenase gene cluster in Sphingobium wenxiniae JZ-1T.Applied and environmental microbiology · 2015Article
- Draft Genome Sequence of Isoproturon-Mineralizing Sphingomonas sp. SRS2, Isolated from an Agricultural Field in the United Kingdom.Genome announcements · 2015Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The environmental fate of phenylurea herbicides has received considerable attention in recent decades. The microbial metabolism of N,N-dimethyl-substituted phenylurea herbicides can generally be initiated by mono-N-demethylation. In this study, the molecular basis for this process was revealed. The pdmAB genes in Sphingobium sp. strain YBL2 were shown to be responsible for the initial mono-N-demethylation of commonly used N,N-dimethyl-substituted phenylurea herbicides. PdmAB is the oxygenase component of a bacterial Rieske non-heme iron oxygenase (RO) system. The genes pdmAB, encoding the α subunit PdmA and the β subunit PdmB, are organized in a transposable element flanked by two direct repeats of an insertion element resembling ISRh1. Furthermore, this transposable element is highly conserved among phenylurea herbicide-degrading sphingomonads originating from different areas of the world. However, there was no evidence of a gene for an electron carrier (a ferredoxin or a reductase) located in the immediate vicinity of pdmAB. Without its cognate electron transport components, expression of PdmAB in Escherichia coli, Pseudomonas putida, and other sphingomonads resulted in a functional enzyme. Moreover, coexpression of a putative [3Fe-4S]-type ferredoxin from Sphingomonas sp. strain RW1 greatly enhanced the catalytic activity of PdmAB in E. coli. These data suggested that PdmAB has a low specificity for electron transport components and that its optimal ferredoxin may be the [3Fe-4S] type. PdmA exhibited low homology to the α subunits of previously characterized ROs (less than 37% identity) and did not cluster with the RO group involved in O- or N-demethylation reactions, indicating that PdmAB is a distinct bacterial RO N-demethylase.
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