ArticleGenesis (New York, N.Y. : 2000)2013
Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis.
Article in Genesis (New York, N.Y. : 2000), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 151 papers.
What it found
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Who cites it
151 citing papers in PubMed, 283 citations in OpenAlex.
- Review
- Molecular Mechanisms of Gonadal Differentiation Induced by 17β-Estradiol and Testosterone Propionate inAnimals : an open access journal from MDPI · 2026Article
- Inbred strains of Xenopus tropicalis show morphological and genetic variation.Scientific reports · 2026Article
- Article
- Transgenesis in Drug Discovery: Enhancing Target Identification and Validation.Molecular biotechnology · 2026Review
- Sex-specific functional evolution of Dmrt1 in African clawed frogs (Xenopus), and the importance of genetic tipping points in developmental biology.PLoS genetics · 2026Article
- TheBiomolecules · 2025Review
- Disrupted endosomal trafficking of the Vangl-Celsr polarity complex underlies congenital anomalies in Xenopus trachea-esophageal morphogenesis.Developmental cell · 2025Article
- Foxh1 is a locus-specific PRC2 recruiter governing germ layer silencing.bioRxiv : the preprint server for biology · 2025Article
- Distinct ossification trade-offs illuminate the shoulder girdle reconfiguration at the water-to-land transition.Nature communications · 2025Article
- Genomic Complexity ofmicroPublication biology · 2025Article
- Intestinal remodeling during Xenopus metamorphosis as a model for studying thyroid hormone signaling and adult organogenesis.Molecular and cellular endocrinology · 2024Article
- Using Xenopus to discover new candidate genes involved in BOR and other congenital hearing loss syndromes.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Review
- Modelling human genetic disorders in Xenopus tropicalis.Disease models & mechanisms · 2024Review
- Simplifying Genotyping of Mutants from Genome Editing with a Parallel qPCR-Based iGenotype Index.Cells · 2024Article
- Functional dissection and assembly of a small, newly evolved, W chromosome-specific genomic region of the African clawed frog Xenopus laevis.PLoS genetics · 2023Article
- Automated multi-sample DNA extraction for genotyping live Xenopus embryos.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- Use of Frogs as a Model to Study the Etiology of HLHS.Journal of cardiovascular development and disease · 2023Article
- Steroid-receptor coactivator complexes in thyroid hormone-regulation of Xenopus metamorphosis.Vitamins and hormones · 2023Article
- Cell cycle activation in thyroid hormone-induced apoptosis and stem cell development duringFrontiers in endocrinology · 2023Review
91 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We have assessed the efficacy of the recently developed CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system for genome modification in the amphibian Xenopus tropicalis. As a model experiment, targeted mutations of the tyrosinase gene were verified, showing the expected albinism phenotype in injected embryos. We further tested this technology by interrupting the six3 gene, which is required for proper eye and brain formation. Expected eye and brain phenotypes were observed when inducing mutations in the six3 coding regions, as well as when deleting the gene promoter by dual targeting. We describe here a standardized protocol for genome editing using this system. This simple and fast method to edit the genome provides a powerful new reverse genetics tool for Xenopus researchers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.