Evidence map›Paper›PMID 24123561›Full record

ArticleGenesis (New York, N.Y. : 2000)2013

A Uchl1-Histone2BmCherry:GFP-gpi BAC transgene for imaging neuronal progenitors.

Carrie B Wiese, Nicole Fleming, Dennis P Buehler, E Michelle Southard-Smith

Open access · greenAbstract readLetter
In one paragraph

Article in Genesis (New York, N.Y. : 2000), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.2field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Carrie B WieseDivision of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine, 529 Light Hall, 2215 Garland Avenue, Nashville, Tennessee.
Nicole Fleming
Dennis P Buehler
E Michelle Southard-Smith
Vanderbilt University · US

Funding

Tumor Immunology and Microenvironment Research ProgramP30CA068485 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI STACIE B DUSETZINA · 1995 to 2026
$172.8M
Translational Analysis CoreP30DK058404 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI RICHARD M. PEEK · 2002 to 2026
$29.9M
Vanderbilt Diabetes Research CenterP30DK020593 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ALVIN C POWERS · 2012 to 2026
$29.3M
TRANSGENIC MOUSE/ ES CELL SHARES RESOURCESP60DK020593 · NIDDK · VANDERBILT UNIVERSITY · PI POWERS, ALVIN C · 1986 to 2011
$28.1M
Shop Module CoreP30EY008126 · NEI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI David J. Calkins · 1989 to 2026
$19.6M
THREE-TIERED PREVENTION MODEL TO BETTER SERVE ALL STUDENP30HD015052 · NICHD · VANDERBILT UNIVERSITY · PI HARRELL, FRANK E. · 1985 to 2014
$18.6M
Vanderbilt Mouse Metabolic Physiology CenterU24DK059637 · NIDDK · VANDERBILT UNIVERSITY · PI WASSERMAN, DAVID H · 2001 to 2015
$14.9M
Vanderbilt Mouse Metabolic Phenotyping CenterU2CDK059637 · NIDDK · VANDERBILT UNIVERSITY · PI WASSERMAN, DAVID H · 2016 to 2021
$6.3M
Murine Atlas of Genitourinary Smooth Muscle DevelopmentU01DK070219 · NIDDK · CINCINNATI CHILDRENS HOSP MED CTR · PI LESSARD, JAMES LOUIS · 2005 to 2009
$4.3M
Gene Networks in Neural Crest-derived Innervation of the Lower Urinary TractRC1DK086594 · NIDDK · VANDERBILT UNIVERSITY · PI SOUTHARD-SMITH, E MICHELLE · 2009 to 2010
$738k
Neural Crest Contributions to the Lower Urinary TractR56DK078158 · NIDDK · VANDERBILT UNIVERSITY · PI SOUTHARD-SMITH, E MICHELLE · 2012 to 2012
$150k
NCI NIH HHS CA68485NCI NIH HHS P30 CA068485NCI NIH HHS P30 CA68485NEI NIH HHS EY08126NEI NIH HHS P30 EY008126NICHD NIH HHS HD15052NICHD NIH HHS P30 HD015052NIDDK NIH HHS DK070219NIDDK NIH HHS DK20593NIDDK NIH HHS DK58404NIDDK NIH HHS DK59637NIDDK NIH HHS P30 DK020593NIDDK NIH HHS P30 DK058404NIDDK NIH HHS P30DK058404NIDDK NIH HHS P60 DK020593NIDDK NIH HHS R56 DK078158NIDDK NIH HHS R56DK078158NIDDK NIH HHS RC1 DK086594NIDDK NIH HHS RC1DK086594NIDDK NIH HHS U01 DK070219NIDDK NIH HHS U01DK070219NIDDK NIH HHS U24 DK059637
6 · The paper itself

Abstract

Uchl1 encodes the protein gene product 9.5 antigen (PGP9.5) that is a widely used to identify migrating neural progenitors in the PNS, mature neurons of the central and peripheral nervous systems, as well as neuroendocrine cells. To facilitate analysis of developing peripheral neurons, we linked regulatory regions of Uchl1 carried within a 160kb bacterial artificial chromosome (BAC) to the dual fluorescent reporter H2BmCherry:GFP-gpi. The Uchl1-H2BmCherry:GFP-gpi transgene exhibits robust expression and allows clear discrimination of individual cells and cellular processes in cranial ganglia, sympathetic chain, the enteric nervous system (ENS), and autonomic ganglia of the urogenital system. The transgene also labels subsets of cells in endocrine tissues where earlier in situ hybridization (ISH) studies have previously identified expression of this deubiquinating enzyme. The Uchl1-H2BmCherry:GFP-gpi transgene will be a powerful tool for static and live imaging, as well as isolation of viable neural progenitors to investigate processes of autonomic neurogenesis.

Indexed as

Chromosomes, Artificial, BacterialMolecular ImagingNeurogenesisAnimalsCell MovementEnteric Nervous SystemFlow CytometryGangliaGenes, ReporterHistonesMiceMice, Inbred C57BLMice, TransgenicMicroscopy, FluorescenceNeuronsRecombinant Fusion ProteinsHistonesRecombinant Fusion ProteinsUbiquitin ThiolesteraseUchl1 protein, mousebacterial artificial chromosome (BAC)enteric nervous systemlower urinary tractneural crestneural progenitorneurogenesisoncogenesisPGP9.5Uchl1

Identifiers

PMID24123561
PMCPMC3953494
OpenAlexW1575044465

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.