ArticleThe Plant cell2013
Nitrogen limitation adaptation, a target of microRNA827, mediates degradation of plasma membrane-localized phosphate transporters to maintain phosphate homeostasis in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers.
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Who cites it
141 citing papers in PubMed, 401 citations in OpenAlex.
- Harnessing microRNA regulatory networks for engineering climate resilience and nutritional enhancement in millets.Stress biology · 2026Review
- The PHO1 protein family in phosphate export and beyond.Plant physiology · 2026Review
- Long-Distance Trafficking of Mineral Elements From Soil-to-Seed Under Climate Change Impacts Crop Nutritional Quality.Plant, cell & environment · 2026Review
- The SPX protein family in plants: from phosphate sensors to multifunctional signaling hubs.Stress biology · 2026Review
- Article
- TaPHL7 Transcription Factor Regulates Utilisation of Nitrogen and Phosphorus in Wheat.Plant biotechnology journal · 2026Article
- Beyond phosphate uptake: PT4/PT11 as key functional components linking phosphate acquisition, nutrient exchange, and arbuscule maintenance in arbuscular mycorrhizal symbiosis.Frontiers in plant science · 2026Review
- Article
- Adaptation mechanisms of low-phosphorus stress in plants: physiological responses, molecular regulation, and future perspectives.Frontiers in plant science · 2026Review
- Glucose-6-Phosphate 1-Epimerase Responds to Phosphate Starvation by Regulating Carbohydrate Homeostasis in Rice andPlants (Basel, Switzerland) · 2025Article
- Rootstock of distant hybrid activates transcription factors of scion cultivar to promote growth and yield in walnut.Plant biotechnology journal · 2025Article
- Overexpression of miR399d impairs arbuscular mycorrhizal symbiosis in tomato.Plant biotechnology journal · 2025Article
- Widespread Presence of SPX and Its Potential Role as a Phosphorus Nutrient Regulator in Dinoflagellates.Microorganisms · 2025Article
- GhSPX1s Interact with GhPHR1A and GhPHL1A in Regulating Phosphate Starvation Response in Cotton.Biology · 2025Article
- Analysing the loss of embryogenic competence in long-term cell lines of Solanum betaceum Cav.: involvement of miR827, phosphate and sugar.BMC plant biology · 2025Article
- The Impact of Nitrogen and Phosphorus Interaction on Growth, Nutrient Absorption, and Signal Regulation in Woody Plants.Biology · 2025Article
- Profiling of Known and Novel microRNAs in an Oleaginous Crop Native to the Amazon Basin, Sacha Inchi (Genes · 2025Article
- Decoding PHR-Orchestrated Stress Adaptation: A Genome-Wide Integrative Analysis of Transcriptional Regulation Under Abiotic Stress inInternational journal of molecular sciences · 2025Article
- MicroRNA gatekeepers: Orchestrating rhizospheric dynamics.Journal of integrative plant biology · 2025Review
- Impact of Nutrient Stress on Plant Disease Resistance.International journal of molecular sciences · 2025Review
81 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the Arabidopsis thaliana phosphate transporter1 (PHT1) family are key players in acquisition of Pi from the rhizosphere, and their regulation is indispensable for the maintenance of cellular Pi homeostasis. Here, we reveal posttranslational regulation of Pi transport through modulation of degradation of PHT1 proteins by the RING-type ubiquitin E3 ligase, nitrogen limitation adaptation (NLA). Loss of function of NLA caused high Pi accumulation resulting from increases in the levels of several PHT1s at the protein rather than the transcript level. Evidence of decreased endocytosis and ubiquitination of PHT1s in nla mutants and interaction between NLA and PHT1s in the plasma membranes suggests that NLA directs the ubiquitination of plasma membrane-localized PHT1s, which triggers clathrin-dependent endocytosis followed by endosomal sorting to vacuoles. Furthermore, different subcellular localization of NLA and phosphate2 (pho2; a ubiquitin E2 conjugase) and the synergistic effect of the accumulation of PHT1s and Pi in nla pho2 mutants suggest that they function independently but cooperatively to regulate PHT1 protein amounts. Intriguingly, NLA and PHO2 are the targets of two Pi starvation-induced microRNAs, miR827 and miR399, respectively. Therefore, our findings uncover modulation of Pi transport activity in response to Pi availability through the integration of a microRNA-mediated posttranscriptional pathway and a ubiquitin-mediated posttranslational regulatory pathway.
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