ArticleExtremophiles : life under extreme conditions2014
Purification and characterization of a novel extracellular halophilic and organic solvent-tolerant amylopullulanase from the haloarchaeon, Halorubrum sp. strain Ha25.
Article in Extremophiles : life under extreme conditions, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 35 citations in OpenAlex.
- Recent Advances in Understanding Bio-Compounds from Haloarchaea.Journal of microbiology and biotechnology · 2026Review
- Heterologous expression and characterization of polyhydroxyalkanoate synthase genes from haloarchaeal strains inFrontiers in microbiology · 2026Article
- Microbial diversity in polyextreme salt flats and their potential applications.Environmental science and pollution research international · 2024Review
- Article
- Bioactive molecules from haloarchaea: Scope and prospects for industrial and therapeutic applications.Frontiers in microbiology · 2023Review
- Genomic attributes of thermophilic and hyperthermophilic bacteria and archaea.World journal of microbiology & biotechnology · 2022Review
- Biotechnological potentials of halophilic microorganisms and their impact on mankind.Beni-Suef University journal of basic and applied sciences · 2022Review
- Halophilic archaea and their potential to generate renewable fuels and chemicals.Biotechnology and bioengineering · 2021Review
- Halophiles and Their Vast Potential in Biofuel Production.Frontiers in microbiology · 2019Review
- Biotechnological applications of archaeal enzymes from extreme environments.Biological research · 2018Review
- Reversible Activation of Halophilic β-lactamase from Methanol-Induced Inactive Form: Contrast to Irreversible Inactivation of Non-Halophilic Counterpart.The protein journal · 2017Article
- Halophiles: biology, adaptation, and their role in decontamination of hypersaline environments.World journal of microbiology & biotechnology · 2016Review
- Characterization of a NaCl-tolerant β-N-acetylglucosaminidase from Sphingobacterium sp. HWLB1.Extremophiles : life under extreme conditions · 2016Article
- Comparison of bacterial biodiversity and enzyme production in three hypersaline lakes; urmia, howz-soltan and aran-bidgol.Indian journal of microbiology · 2014Article
- Effect of organic solvents on the structure and activity of moderately halophilic Bacillus sp. EMB9 protease.Extremophiles : life under extreme conditions · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A halophilic archaeon, Halorubrum sp. strain Ha25, produced extracellular halophilic organic solvent-tolerant amylopullulanase. The maximum enzyme production was at high salt concentration, 3-4 M NaCl. Optimum pH and temperature for enzyme production were 7.0 and 40 °C, respectively. Molecular mass of purified enzyme was estimated to be about 140 kDa by SDS-PAGE. This enzyme was active on pullulan and starch as substrates. The apparent Km for the enzyme activity on pullulan was 4 mg/ml and for soluble starch was 1.8 mg/ml. Optimum temperature for amylolytic and pullulytic activities was 50 °C. Optimum pH for amylolytic activity was 7 and for pullulytic activity was 7.5. This enzyme was active over a wide range of concentrations (0-4.5 M) of NaCl. The effect of organic solvents on the enzyme activities showed that this enzyme was more stable in the presence of non-polar organic solvents than polar solvents. This study is the first report on amylopullulanase production in halophilic bacteria and archaea.
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