ArticleNature methods2013
Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells.
Article in Nature methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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Who cites it
91 citing papers in PubMed, 253 citations in OpenAlex.
- Non-diffusive slow heat dissipation induces high local temperature in living cells.Nature communications · 2026Article
- Transient Plasmonic Photothermal Imaging Deciphers Dynamic Thermal Transfer during the Cell Cycle.Chemical & biomedical imaging · 2026Article
- Optically Detected Magnetic Resonance Based Intracellular Thermometry Using Nanodiamonds Implanted in Adherent Cancer Cells.ACS applied nano materials · 2026Article
- Chemically Anchored Diamond with H3 Centers for Ratiometric Measurement of Isolated Mitochondria Temperature.International journal of molecular sciences · 2025Article
- Alteration of metabolic activity regulates mitochondrial temperature in diagnosis in HepG2 hepatocellular carcinoma cells.Scientific reports · 2025Article
- Single-Molecule Vibrational Thermometry.The journal of physical chemistry. B · 2025Article
- Multiplexed quantum sensing reveals coordinated thermomagnetic regulation of mitochondria.bioRxiv : the preprint server for biology · 2025Article
- Genetically-encoded temperature indicators for thermal biology.Biophysics and physicobiology · 2025Article
- Heat application in live cell imaging.FEBS open bio · 2024Review
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Multimodal Temperature Readout Boosts the Performance of CuInSACS applied materials & interfaces · 2024Article
- TRPV2: a universal regulator in cellular physiology with a yet poorly defined thermosensitivity.The journal of physiological sciences : JPS · 2024Review
- Extremely Sensitive Genetically Encoded Temperature Indicator Enabling Measurement at the Organelle Level.ACS sensors · 2024Article
- Physicochemical Perspective of Biological Heterogeneity.ACS physical chemistry Au · 2024Review
- Continuous spatial field confocal thermometry using lanthanide doped tellurite glass.Scientific reports · 2024Article
- Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism.Annual review of biophysics · 2024Review
- Tutorial on compressed ultrafast photography.Journal of biomedical optics · 2024Review
- Limitations of Bulk Diamond Sensors for Single-Cell Thermometry.Sensors (Basel, Switzerland) · 2023Article
- X-Ray Nanothermometry of Nanoparticles in Tumor-Mimicking Tissues under Photothermia.Advanced healthcare materials · 2023Article
- Warm Cells, Hot Mitochondria: Achievements and Problems of Ultralocal Thermometry.International journal of molecular sciences · 2023Review
31 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammals and birds, thermoregulation to conserve body temperature is vital to life. Multiple mechanisms of thermogeneration have been proposed, localized in different subcellular organelles. However, visualizing thermogenesis directly in intact organelles has been challenging. Here we have developed genetically encoded, GFP-based thermosensors (tsGFPs) that enable visualization of thermogenesis in discrete organelles in living cells. In tsGFPs, a tandem formation of coiled-coil structures of the Salmonella thermosensing protein TlpA transmits conformational changes to GFP to convert temperature changes into visible and quantifiable fluorescence changes. Specific targeting of tsGFPs enables visualization of thermogenesis in the mitochondria of brown adipocytes and the endoplasmic reticulum of myotubes. In HeLa cells, tsGFP targeted to mitochondria reveals heterogeneity in thermogenesis that correlates with the electrochemical gradient. Thus, tsGFPs are powerful tools to noninvasively assess thermogenesis in living cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.