ArticleActa pharmacologica Sinica2014
Age-dependent contribution of Rho kinase in carbachol-induced contraction of human detrusor smooth muscle in vitro.
Article in Acta pharmacologica Sinica, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 15 citations in OpenAlex.
- Characterization of calcium sources and downstream signaling pathways following electrical field stimulation of rodent longitudinal esophageal muscle.Frontiers in physiology · 2026Article
- Age-dependent effects of the βPflugers Archiv : European journal of physiology · 2024Article
- Intracellular signaling pathways of muscarinic acetylcholine receptor-mediated detrusor muscle contractions.American journal of physiology. Renal physiology · 2023Article
- Ageing influences detrusor contractions to prostaglandin, angiotensin, histamine and 5-HT (serotonin), independent to the Rho kinase and extracellular calcium pathways.Scientific reports · 2023Article
- Article
- Signaling Pathways Mediating Bradykinin-Induced Contraction in Murine and Human Detrusor Muscle.Frontiers in medicine · 2021Article
- Inverse relationship of Rho kinase and myosin-light chain kinase expression in the aging human detrusor smooth muscle.BMC urology · 2015Article
- Aging changes agonist induced contractile responses in permeabilized rat bladder.Age (Dordrecht, Netherlands) · 2015Article
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6 authors at 1 institution in 1 country.
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Abstract
aimActivation of muscarinic receptors on the detrusor smooth muscle is followed by contraction, which involves both myosin light chain kinase (MLCK) and Rho kinase (ROCK). The aim of this study was to determine the relative contributions of MLCK and ROCK to carbachol-induced contraction of human detrusor smooth muscle in vitro.
methodsDetrusor smooth muscle strips were prepared from the macroscopically unaffected bladder wall of patients underwent cystectomy. The strips were fixed in an organ bath, and carbachol or KCl-induced isometric contractions were measured by force transducers.
resultsAddition of carbachol (0.4-4 μmol/L) into the bath induced concentration-dependent contractions of detrusor specimens, which was completely abolished by atropine (1 μmol/L). Pre-incubation of detrusor specimens with either the MLCK inhibitor ML-9 or the ROCK inhibitors HA1100 and Y-27632 (each at 10 μmol/L) significantly blocked carbachol-induced contractions as compared to the time-control experiments. Moreover, MLCK and ROCK inhibition were equally effective in reducing carbachol-induced contractions. The residual carbachol-induced contractions in the presence of both MLCK and ROCK inhibitors were significantly smaller than the contractions obtained when only one enzyme (either MLCK or ROCK) was inhibited, suggesting an additive effect of the two kinases. Interestingly, ROCK-mediated carbachol-induced contractions were positively correlated to the age of patients (r=o.52, P<0.05).
conclusionBoth MLCK and ROCK contribute to carbachol-induced contractions of human detrusor smooth muscle. ROCK inhibitors may be a new pharmacological approach to modulate human bladder hyperactivity.
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