ArticleApplied microbiology and biotechnology2014
Bacterial chitinase with phytopathogen control capacity from suppressive soil revealed by functional metagenomics.
Article in Applied microbiology and biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Microbial chitinases in the plant holobiont: ecological roles and structural determinants.FEMS microbiology reviews · 2026Review
- Improved Expression of a Thermostable GH18 Bacterial Chitinase in Two Different Escherichia coli Strains and Its Potential Use in Plant Protection and Biocontrol of Phytopathogenic Fungi.Molecular biotechnology · 2024Article
- Transplantation of soil from organic field confers disease suppressive ability to conducive soil.World journal of microbiology & biotechnology · 2024Article
- Functional Comparison of Three Chitinases from Symbiotic Bacteria of Entomopathogenic Nematodes.Toxins · 2024Article
- An overview of viral chitinases: General properties and biotechnological potential.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- Role of Fungi in Imparting General Disease Suppressiveness in Soil from Organic Field.Microbial ecology · 2023Article
- Giant Viruses as a Source of Novel Enzymes for Biotechnological Application.Pathogens (Basel, Switzerland) · 2022Review
- Potential Use of Microbial Community Genomes in Various Dimensions of Agriculture Productivity and Its Management: A Review.Frontiers in microbiology · 2022Review
- Antagonistic StrainPathogens (Basel, Switzerland) · 2021Article
- Effects of long-term integrated agri-aquaculture on the soil fungal community structure and function in vegetable fields.Scientific reports · 2021Article
- Functional Metagenomic Screening for Antimicrobial Resistance in the Oral Microbiome.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Expression and characterization of two chitinases with synergistic effect and antifungal activity from Xenorhabdus nematophila.World journal of microbiology & biotechnology · 2019Article
- Stenotrophomonas maltophilia AHL-Degrading Strains Isolated from Marine Invertebrate Microbiota Attenuate the Virulence of Pectobacterium carotovorum and Vibrio coralliilyticus.Marine biotechnology (New York, N.Y.) · 2019Article
- Recovery and functional validation of hidden soil enzymes in metagenomic libraries.MicrobiologyOpen · 2019Article
- Metagenome-Sourced Microbial Chitinases as Potential Insecticide Proteins.Frontiers in microbiology · 2019Article
- Chitinase: diversity, limitations, and trends in engineering for suitable applications.Bioscience reports · 2018Review
- Article
- Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome.Microbial cell factories · 2017Article
- Current Insights into the Role of Rhizosphere Bacteria in Disease Suppressive Soils.Frontiers in microbiology · 2017Review
- The rhizospheric microbial community structure and diversity of deciduous and evergreen forests in Taihu Lake area, China.PloS one · 2017Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Plant disease caused by fungal pathogens results in vast crop damage globally. Microbial communities of soil that is suppressive to fungal crop disease provide a source for the identification of novel enzymes functioning as bioshields against plant pathogens. In this study, we targeted chitin-degrading enzymes of the uncultured bacterial community through a functional metagenomics approach, using a fosmid library of a suppressive soil metagenome. We identified a novel bacterial chitinase, Chi18H8, with antifungal activity against several important crop pathogens. Sequence analyses show that the chi18H8 gene encodes a 425-amino acid protein of 46 kDa with an N-terminal signal peptide, a catalytic domain with the conserved active site F175DGIDIDWE183, and a chitinase insertion domain. Chi18H8 was expressed (pGEX-6P-3 vector) in Escherichia coli and purified. Enzyme characterization shows that Chi18H8 has a prevalent chitobiosidase activity with a maximum activity at 35 °C at pH lower than 6, suggesting a role as exochitinase on native chitin. To our knowledge, Chi18H8 is the first chitinase isolated from a metagenome library obtained in pure form and which has the potential to be used as a candidate agent for controlling fungal crop diseases. Furthermore, Chi18H8 may also answer to the demand for novel chitin-degrading enzymes for a broad range of other industrial processes and medical purposes.
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