ArticleScientific reports2013
Direct confocal acquisition of fluorescence from X-gal staining on thick tissue sections.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
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- FTO promotes weight gain via altering Kif1a splicing and axonal vesicle trafficking in AgRP neurons.The EMBO journal · 2025Article
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- Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice.Nature communications · 2022Article
- Stress-induced premature senescence is associated with a prolonged QT interval and recapitulates features of cardiac aging.Theranostics · 2022Article
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- Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord.Frontiers in cell and developmental biology · 2021Article
- The unique expression profile of FAM19A1 in the mouse brain and its association with hyperactivity, long-term memory and fear acquisition.Scientific reports · 2020Article
- Cortical neurons develop a senescence-like phenotype promoted by dysfunctional autophagy.Aging · 2019Article
- FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain ofFrontiers in neuroscience · 2019Article
- Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos.Journal of visualized experiments : JoVE · 2018Article
- Absence of CFAP69 Causes Male Infertility due to Multiple Morphological Abnormalities of the Flagella in Human and Mouse.American journal of human genetics · 2018Article
- Comparison of proliferative and immunomodulatory potential of adipose-derived mesenchymal stem cells from young and geriatric cats.Journal of feline medicine and surgery · 2017Article
- Definitive Hematopoiesis in the Yolk Sac Emerges from Wnt-Responsive Hemogenic Endothelium Independently of Circulation and Arterial Identity.Stem cells (Dayton, Ohio) · 2016Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
X-gal staining is a common procedure used in the histochemical monitoring of gene expression by light microscopy. However, this procedure does not permit the direct confocal acquisition of images, thus preventing the identification of labelled cells on the depth (Z) axis of tissue sections and leading sometimes to erroneous conclusions in co-localization and gene expression studies. Here we report a technique, based on X-gal fluorescence emission and mathematically-based optical correction, to obtain high quality fluorescence confocal images. This method, combined with immunofluorescence, makes it possible to unequivocally identify X-gal-labelled cells in tissue sections, emerging as a valuable tool in gene expression and cell tracing analysis.
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