ArticleGenetics2013
Genetically engineered transvestites reveal novel mating genes in budding yeast.
Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Evolutionary responses to increased opportunity for sexual selection in yeast.BMC ecology and evolution · 2026Article
- Global Transcriptomic Analysis Identifies Gene Expression Programs Regulated by α-Factor in Yeast.Journal of microbiology and biotechnology · 2025Article
- A yeast surface display platform for characterizing CAR T cell responses to cancer antigens.Nature communications · 2025Article
- Evolutionary responses to increased opportunity for sexual selection in yeast.bioRxiv : the preprint server for biology · 2025Article
- A yeast mating platform for multiplex screening of fungal GPCR-ligand interactions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts.Nature communications · 2022Article
- Functional Divergence in a Multi-gene Family Is a Key Evolutionary Innovation for Anaerobic Growth in Saccharomyces cerevisiae.Molecular biology and evolution · 2022Article
- Mechanism of commitment to a mating partner inMolecular biology of the cell · 2022Article
- Pheromone Guidance of Polarity Site Movement in Yeast.Biomolecules · 2022Article
- Selecting for Altered Substrate Specificity Reveals the Evolutionary Flexibility of ATP-Binding Cassette Transporters.Current biology : CB · 2020Article
- Unisexual reproduction promotes competition for mating partners in the global human fungal pathogen Cryptococcus deneoformans.PLoS genetics · 2019Article
- Identification and characterization of the Komagataella phaffii mating pheromone genes.FEMS yeast research · 2018Article
- Sensory input attenuation allows predictive sexual response in yeast.Nature communications · 2016Article
- Cell biology of yeast zygotes, from genesis to budding.Biochimica et biophysica acta · 2015Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Haploid budding yeast has two mating types, defined by the alleles of the MAT locus, MATa and MATα. Two haploid cells of opposite mating types mate by signaling to each other using reciprocal pheromones and receptors, polarizing and growing toward each other, and eventually fusing to form a single diploid cell. The pheromones and receptors are necessary and sufficient to define a mating type, but other mating-type-specific proteins make mating more efficient. We examined the role of these proteins by genetically engineering "transvestite" cells that swap the pheromone, pheromone receptor, and pheromone processing factors of one mating type for another. These cells mate with each other, but their mating is inefficient. By characterizing their mating defects and examining their transcriptomes, we found Afb1 (a-factor barrier), a novel MATα-specific protein that interferes with a-factor, the pheromone secreted by MATa cells. Strong pheromone secretion is essential for efficient mating, and the weak mating of transvestites can be improved by boosting their pheromone production. Synthetic biology can characterize the factors that control efficiency in biological processes. In yeast, selection for increased mating efficiency is likely to have continually boosted pheromone levels and the ability to discriminate between partners who make more and less pheromone. This discrimination comes at a cost: weak mating in situations where all potential partners make less pheromone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.