ArticleJournal of visualized experiments : JoVE2013
Intraductal injection for localized drug delivery to the mouse mammary gland.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Comparative oncogenomics identifies combinations of driver genes and drug targets in BRCA1-mutated breast cancer.Nature communications · 2019Pooled it
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- OBSERVE: guidelines for the refinement of rodent cancer models.Nature protocols · 2024Review
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- Thermosensitive Polymeric Nanoparticles for Drug Co-Encapsulation and Breast Cancer Treatment.Pharmaceutics · 2024Article
- Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models.Journal of visualized experiments : JoVE · 2022Article
- Merlin deficiency alters the redox management program in breast cancer.Molecular oncology · 2021Article
- Truncated ASPP2 Drives Initiation and Progression of Invasive Lobular Carcinoma via Distinct Mechanisms.Cancer research · 2020Article
- In situ CRISPR-Cas9 base editing for the development of genetically engineered mouse models of breast cancer.The EMBO journal · 2020Article
- Ductal tree ablation by local delivery of ethanol prevents tumor formation in an aggressive mouse model of breast cancer.Breast cancer research : BCR · 2019Article
- Tissue-specific microRNA expression alters cancer susceptibility conferred by a TP53 noncoding variant.Nature communications · 2019Article
- Multifunctional nanoemulsions for intraductal delivery as a new platform for local treatment of breast cancer.Drug delivery · 2018Article
- Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation.Nature communications · 2018Article
- Heterochromatin-Encoded Satellite RNAs Induce Breast Cancer.Molecular cell · 2018Article
- Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology.Journal of visualized experiments : JoVE · 2017Article
- Insertional mutagenesis identifies drivers of a novel oncogenic pathway in invasive lobular breast carcinoma.Nature genetics · 2017Article
- MRI ductography of contrast agent distribution and leakage in normal mouse mammary ducts and ducts with in situ cancer.Magnetic resonance imaging · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of genes within the mammary epithelium has been difficult to achieve due to the lack of appropriate targeting molecules that are independent of developmental stages such as pregnancy and lactation. Herein, we describe a technique for localized delivery of reagents to the mammary gland at any stage in adulthood via intraductal injection into the nipples of mice. The injections can be performed on live mice, under anesthesia, and allow for a non-invasive and localized drug delivery to the mammary gland. Furthermore, the injections can be repeated over several months without damaging the nipple. Vital dyes such as Evans Blue are very helpful to learn the technique. Upon intraductal injection of the blue dye, the entire ductal tree becomes visible to the eye. Furthermore, fluorescently labeled reagents also allow for visualization and distribution within the mammary gland. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents, and small molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.