ArticleNucleic acids research2014
Conformational and thermodynamic hallmarks of DNA operator site specificity in the copper sensitive operon repressor from Streptomyces lividans.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Regulation of a nickel tolerance operon conserved inApplied and environmental microbiology · 2025Article
- Article
- A CsoR family transcriptional regulator, TTHA1953, controls the sulfur oxidation pathway in Thermus thermophilus HB8.The Journal of biological chemistry · 2023Article
- Role of horizontally transferred copper resistance genes inMicrobiology (Reading, England) · 2022Review
- Functional asymmetry and chemical reactivity of CsoR family persulfide sensors.Nucleic acids research · 2021Article
- Genetic Regulation of Metal Ion Homeostasis in Staphylococcus aureus.Trends in microbiology · 2020Review
- Allosteric control of metal-responsive transcriptional regulators in bacteria.The Journal of biological chemistry · 2020Review
- Complexation of the nickel and cobalt transcriptional regulator RcnR with DNA.Acta crystallographica. Section F, Structural biology communications · 2020Article
- A tight tunable range for Ni(II) sensing and buffering in cells.Nature chemical biology · 2017Article
- Pb2+ tolerance by Frankia sp. strain EAN1pec involves surface-binding.Microbiology (Reading, England) · 2017Article
- The mechanism of a formaldehyde-sensing transcriptional regulator.Scientific reports · 2016Article
- Game of 'Somes: Protein Destruction for Mycobacterium tuberculosis Pathogenesis.Trends in microbiology · 2016Review
- Resolution of Stepwise Cooperativities of Copper Binding by the Homotetrameric Copper-Sensitive Operon Repressor (CsoR): Impact on Structure and Stability.Angewandte Chemie (International ed. in English) · 2015Article
- Mycobacterium tuberculosis and Copper: A Newly Appreciated Defense against an Old Foe?The Journal of biological chemistry · 2015Review
- Cu(I)-mediated allosteric switching in a copper-sensing operon repressor (CsoR).The Journal of biological chemistry · 2014Article
- Metal specificity of cyanobacterial nickel-responsive repressor InrS: cells maintain zinc and copper below the detection threshold for InrS.Molecular microbiology · 2014Article
- Recent developments in copper and zinc homeostasis in bacterial pathogens.Current opinion in chemical biology · 2014Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metal ion homeostasis in bacteria relies on metalloregulatory proteins to upregulate metal resistance genes and enable the organism to preclude metal toxicity. The copper sensitive operon repressor (CsoR) family is widely distributed in bacteria and controls the expression of copper efflux systems. CsoR operator sites consist of G-tract containing pseudopalindromes of which the mechanism of operator binding is poorly understood. Here, we use a structurally characterized CsoR from Streptomyces lividans (CsoR(Sl)) together with three specific operator targets to reveal the salient features pertaining to the mechanism of DNA binding. We reveal that CsoR(Sl) binds to its operator site through a 2-fold axis of symmetry centred on a conserved 5'-TAC/GTA-3' inverted repeat. Operator recognition is stringently dependent not only on electropositive residues but also on a conserved polar glutamine residue. Thermodynamic and circular dichroic signatures of the CsoR(Sl)-DNA interaction suggest selectivity towards the A-DNA-like topology of the G-tracts at the operator site. Such properties are enhanced on protein binding thus enabling the symmetrical binding of two CsoR(Sl) tetramers. Finally, differential binding modes may exist in operator sites having more than one 5'-TAC/GTA-3' inverted repeat with implications in vivo for a mechanism of modular control.
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