ArticleNature communications2013
Zinc-finger nickase-mediated insertion of the lysostaphin gene into the beta-casein locus in cloned cows.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 108 citations in OpenAlex.
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- Emerging Roles of Noncoding RNAs in Bovine Mastitis Diseases.Pathogens (Basel, Switzerland) · 2022Review
- Application of Gene Editing Technology in Resistance Breeding of Livestock.Life (Basel, Switzerland) · 2022Review
- Evaluation of the α-casein (CSN1S1) locus as a potential target for a site-specific transgene integration.Scientific reports · 2022Article
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- Secretion of IFN-γ by Transgenic Mammary Epithelial CellsFrontiers in veterinary science · 2022Article
- Impact of CRISPR-Cas9-Based Genome Engineering in Farm Animals.Veterinary sciences · 2021Review
- Extranuclear Inheritance of Mitochondrial Genome and Epigenetic Reprogrammability of Chromosomal Telomeres in Somatic Cell Cloning of Mammals.International journal of molecular sciences · 2021Review
- Genomic Analysis, Progress and Future Perspectives in Dairy Cattle Selection: A Review.Animals : an open access journal from MDPI · 2021Review
- Histone deacetylases inhibitor and RAD51 recombinase increase transcription activator-like effector nucleases-mediated homologous recombination on the bovine β-casein gene locus.Asian-Australasian journal of animal sciences · 2020Article
- Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts.BMC molecular and cell biology · 2020Article
- Application of genome editing in farm animals: cattle.Transgenic research · 2019Article
- Harnessing genomic information for livestock improvement.Nature reviews. Genetics · 2019Review
- Genome editing for the treatment of tumorigenic viral infections and virus-related carcinomas.Frontiers of medicine · 2018Review
- Development of genome engineering technologies in cattle: from random to specific.Journal of animal science and biotechnology · 2018Review
- Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle.Scientific reports · 2017Article
- Genome editing in livestock: Are we ready for a revolution in animal breeding industry?Transgenic research · 2017Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zinc-finger nickases (ZFNickases) are a type of programmable nuclease that can be engineered from zinc-finger nucleases to induce site-specific single-strand breaks or nicks in genomic DNA, which result in homology-directed repair. Although zinc-finger nuclease-mediated gene disruption has been demonstrated in pigs and cattle, they have not been used to target gene addition into an endogenous gene locus in any large domestic species. Here we show in bovine fetal fibroblasts that targeting ZFNickases to the endogenous β-casein (CSN2) locus stimulates lysostaphin gene addition by homology-directed repair. We find that ZFNickase-treated cells can be successfully used in somatic cell nuclear transfer, resulting in live-born gene-targeted cows. Furthermore, the gene-targeted cows secrete lysostaphin in their milk and in vitro assays demonstrate the milk's ability to kill Staphylococcus aureus. Our success with this strategy will facilitate new transgenic technologies beneficial to both agriculture and biomedicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.