ArticleThe Journal of investigative dermatology2014
Crosstalk between keratinocytes and T cells in a 3D microenvironment: a model to study inflammatory skin diseases.
Article in The Journal of investigative dermatology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
75 citing papers in PubMed, 159 citations in OpenAlex.
- Recent advances in immunocompetent human skin-on-chip models: Construction strategies and biomedical applications.Bioactive materials · 2026Review
- Potential of 3D Skin Models and N/TERT-2G Cell Line in Genetic Research on Autosomal Recessive Nonsyndromic Epidermal Differentiation Disorders.Experimental dermatology · 2026Article
- A review of 3D In vitro skin models for psoriasis: from pathophysiology to advanced bioengineering.Frontiers in bioengineering and biotechnology · 2026Review
- CD4Frontiers in immunology · 2026Article
- Toward system-level integration of organoids for regenerative medicine.Burns & trauma · 2026Review
- High-Throughput 3D Bioprinted Organoids of Skin Cancer Utilized for Diagnosis and Personalized Therapy.Current oncology (Toronto, Ont.) · 2025Review
- A hair-follicle reconstructed in vitro immunocompetent skin model for prediction of the sensitizing potential of chemicals.Archives of toxicology · 2025Article
- Toward the Next Generation of In Silico Modeling of Dynamic Host-Microbiota Interactions in the Skin.JID innovations : skin science from molecules to population health · 2025Review
- Marek's disease virus replication in chicken skin reconstructedThe Journal of general virology · 2025Article
- Replicating Dynamic Immune Responses at Single-Cell Resolution within a Microfluidic Human Skin Equivalent.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Enhancing Transcutaneous Drug Delivery: Advanced Perspectives on Skin Models.JID innovations : skin science from molecules to population health · 2025Review
- IL-1 signaling enrichment in inflammatory skin disease loci with higher-risk allele frequencies in African ancestry.Research square · 2025Article
- Development of pathological skin models: from conventional techniques to 3D bioprinting.Frontiers in bioengineering and biotechnology · 2025Review
- Beyond the dichotomy: understanding the overlap between atopic dermatitis and psoriasis.Frontiers in immunology · 2025Review
- Engineering a 3D wounded skin equivalent to study early inflammatory and regenerative responsesFrontiers in bioengineering and biotechnology · 2025Article
- Breakthrough Opportunities of Nanotheranostics in Psoriasis: From Pathogenesis to Management Strategy.Infectious disorders drug targets · 2025Review
- A standardizable human-based psoriasis skin model for drug development.Frontiers in medicine · 2025Article
- Development of immunocompetent full thickness skin tissue constructs to model skin fibrosis for high-throughput drug screening.Biofabrication · 2024Article
- Diversity of human skin three-dimensional organotypic cultures.Current opinion in genetics & development · 2024Review
- Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction between keratinocytes and immune cells plays a major role in the development of inflammatory skin diseases like psoriasis and atopic dermatitis. Pharmacological intervention to inhibit T cell-derived proinflammatory mediators is an effective therapy in the treatment of psoriasis. Here, we present a model to study the interaction between keratinocytes and T cells in a three-dimensional (3D) microenvironment, based on human skin equivalents populated with CD4+ T cells. T cell migration into the dermis initiated keratinocyte activation within 2 days, with hallmarks of a psoriasiform inflammation after 4 days. Expression of epidermal psoriasis marker genes was upregulated, and proinflammatory cytokines and chemokines were highly expressed. Disturbed epidermal differentiation was shown by downregulated filaggrin expression and involucrin expression in the spinous layer. These effects were mediated via soluble factors produced by the T cells. The psoriasiform inflammation was also observed using T helper type 1 (Th1)- and Th17-polarized CD4+ T cells. We validated our model by treatment with anti-inflammatory drugs that reduced the expression of proinflammatory cytokines and chemokines and suppressed the psoriasiform inflammation. We propose that our T cell-driven inflammatory skin equivalent model has potential to study the pathogenesis of inflammatory skin diseases and may serve as a preclinical screening tool for anti-inflammatory drugs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.